A new in vitro model for agonist-induced communication between microvascular endothelial cells

Yves Ouellette, Darcy Lidington, Christian G. Naus, Karel Tyml

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Microvascular endothelial cells (MECs) grown in Matrigel form capillary-like structures. We hypothesized that these 'capillaries' better mimic communication properties of microvessels than conventional cell monolayers. MECs were isolated from the rat hindlimb skeletal muscle. Functional communication was tested by visualizing the spread of microinjected 6-carboxyfluorescein (CF) dye and by measuring a conducted change of membrane potential after micropipette application of 500 mM KCl or 10 mM adenosine triphosphate (ATP) on the capillary and monolayer. MECs grown under both conditions were dye-coupled, as demonstrated by the spread of CF injected into a single cell. The membrane potential of cells grown in capillaries (-59 ± 5 mV) was significantly greater than that of cells grown in monolayers (-24 ± 2 mV). KCl and ATP caused local depolarization (18 ± 3 mV) and hyperpolarization (21 ± 3 mV) in capillaries that yielded conducted 13 ± 3 mV depolarization and 15 ± 5 mV hyperpolarization at a 300-μm distal site, respectively. In monolayers, local and distal responses to agonists were 3- to 6-fold and 9- to 10-fold less, respectively, than the corresponding responses in capillaries. Cells grown under both conditions expressed connexin 43, as demonstrated by immunohistochemistry and Western blotting. We conclude that cells grown in capillaries yield substantially larger local and communicated responses than cells in monolayers and thus offer a more sensitive model for mechanistic studies of MEC communication. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)222-231
Number of pages10
JournalMicrovascular Research
Volume60
Issue number3
DOIs
StatePublished - Jan 1 2000
Externally publishedYes

Fingerprint

Endothelial cells
Monolayers
Endothelial Cells
Communication
Cells
Depolarization
Coloring Agents
Adenosine Triphosphate
Membrane Potentials
Membranes
Connexin 43
Muscle
Rats
Hindlimb
Microvessels
In Vitro Techniques
Cell Communication
Skeletal Muscle
Western Blotting
Immunohistochemistry

Keywords

  • Communication
  • Endothelial cell
  • Gap junctions
  • Skeletal muscle

ASJC Scopus subject areas

  • Biochemistry
  • Cardiology and Cardiovascular Medicine
  • Cell Biology

Cite this

A new in vitro model for agonist-induced communication between microvascular endothelial cells. / Ouellette, Yves; Lidington, Darcy; Naus, Christian G.; Tyml, Karel.

In: Microvascular Research, Vol. 60, No. 3, 01.01.2000, p. 222-231.

Research output: Contribution to journalArticle

Ouellette, Yves ; Lidington, Darcy ; Naus, Christian G. ; Tyml, Karel. / A new in vitro model for agonist-induced communication between microvascular endothelial cells. In: Microvascular Research. 2000 ; Vol. 60, No. 3. pp. 222-231.
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