A new genetic method to generate and isolate small, short-lived but highly potent dendritic cell-tumor cell hybrid vaccines

Vy Phan, Fiona Errington, S. Chiat Cheong, Tim Kottke, Michael Gough, Sharon Altmann, Annick Brandenburger, Steve Emery, Scott Strome, Andrew Bateman, Bernard Bonnotte, Alan Melcher, Richard Vile

Research output: Contribution to journalArticlepeer-review

66 Scopus citations

Abstract

Fusion of tumor cells with antigen-presenting cells (APCs) has been proposed for the preparation of cancer vaccines. However, generation of these hybrids, using physical or chemical methods such as electrofusion or polyethylene glycol (PEG), has been difficult to standardize. Characterization of cell fusion has also been problematic because of difficulties in differentiating fusion from cell aggregation, leakage of cellular dyes and dendritic-cell (DC) phagocytosis of tumor material. In this report, we describe a new method to generate hybrid cell vaccines, based on gene transfer of a viral fusogenic membrane glycoprotein (FMG) into tumor cells, and incorporate a genetic method by which true hybrid formation can be unambiguously detected. We describe a new class of tumor cell-DC hybrid that can be rapidly isolated after cell fusion. These hybrids are highly potent in in vitro antigen presentation assays, target lymph nodes in vivo and are powerful immunogens against established metastatic disease.

Original languageEnglish (US)
Pages (from-to)1215-1219
Number of pages5
JournalNature Medicine
Volume9
Issue number9
DOIs
StatePublished - Sep 1 2003

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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