A New Approach to Assess the Gastrocnemius Muscle Volume in Rodents Using Ultrasound; Comparison with the Gastrocnemius Muscle Index

Tim H J Nijhuis, Siebe A S de Boer, Abhijeet L. Wahegaonkar, Allen Thorp Bishop, Alexander Yong-Shik Shin, Steven E R Hovius, Ruud W. Selles

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Introduction: The purpose of this study was to determine the reliability and validity of a new non-invasive ultrasound technique to measure gastrocnemius muscle atrophy after nerve denervation in an animal model. Methods: In sixteen rodents an eight mm sciatic nerve gap was created. In the following 8 weeks, each week, two rodents were euthanized and the gastrocnemius muscle was examined using two different ultrasound systems and two investigators. The standardized ultrasound measurement protocol consisted of identifying pre-defined anatomical landmarks: 1) the fibula, 2) the fibular nerve, and 3) the junction between the most distal point of the semitendinosus muscle and gastrocnemius muscle. Consequently, we measured the muscle thickness as the length of the line between the fibula and the junction between the two muscles, perpendicular to the fibular nerve. After the ultrasound recording, the muscle mass was determined. Results: A steep decline of muscle weight of 24% was observed after one week. In the following weeks, the weight further decreased and then remained stable from 6 weeks onwards, resulting in a maximal muscle weight decrease of 82%. The correlation coefficient was >0.96 between muscle diameter and weight using both ultrasound systems. The inter-rater reliability was excellent for both devices on the operated side (ICC of 0.99 for both ultrasound systems) and good for the non-operated site (ICC's: 0.84 & 0.89). The difference between the muscle mass ratio and the muscle thickness ratio was not more than 5% with two outliers of approximately 13%. Discussion: We have developed an innovative, highly reliable technique for quantifying muscle atrophy after nerve injury. This technique allows serial measurements in the same animal over time. This is a significant advantage compared to the conventional technique for quantifying muscle atrophy, which requires sacrificing the animal.

Original languageEnglish (US)
Article numbere54041
JournalPLoS One
Volume8
Issue number1
DOIs
StatePublished - Jan 17 2013

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Muscle
Rodentia
Skeletal Muscle
rodents
Ultrasonics
Muscles
muscles
nerve tissue
Muscular Atrophy
muscular atrophy
Weights and Measures
fibula
Peroneal Nerve
Fibula
Animals
semitendinosus muscle
methodology
Denervation
Sciatic Nerve
Reproducibility of Results

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

A New Approach to Assess the Gastrocnemius Muscle Volume in Rodents Using Ultrasound; Comparison with the Gastrocnemius Muscle Index. / Nijhuis, Tim H J; de Boer, Siebe A S; Wahegaonkar, Abhijeet L.; Bishop, Allen Thorp; Shin, Alexander Yong-Shik; Hovius, Steven E R; Selles, Ruud W.

In: PLoS One, Vol. 8, No. 1, e54041, 17.01.2013.

Research output: Contribution to journalArticle

Nijhuis, Tim H J ; de Boer, Siebe A S ; Wahegaonkar, Abhijeet L. ; Bishop, Allen Thorp ; Shin, Alexander Yong-Shik ; Hovius, Steven E R ; Selles, Ruud W. / A New Approach to Assess the Gastrocnemius Muscle Volume in Rodents Using Ultrasound; Comparison with the Gastrocnemius Muscle Index. In: PLoS One. 2013 ; Vol. 8, No. 1.
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