TY - JOUR
T1 - A multicenter investigation with interphase fluorescence in situ hybridization using X- and Y-chromosome probes
AU - Dewald, Gordon
AU - Stallard, Richard
AU - Saadi, A. A.I.
AU - Arnold, Susan
AU - Bader, Patricia I.
AU - Blough, Ruthann
AU - Chen, Kathy
AU - Rafaël Elejalde, B.
AU - Harris, Catherine J.
AU - Higgins, Rodney R.
AU - Hoeltge, Gerald A.
AU - Hsu, Wei Tong
AU - Kubic, Virginia
AU - James McCorquodale, D.
AU - Micale, Mark A.
AU - Moore, J. W.
AU - Phillips, Rosalie M.
AU - Scheib-Wixted, Susan
AU - Schwartz, Stuart
AU - Siembieda, Steven
AU - Strole, Kathy
AU - Vantuinen, Peter
AU - Vance, Gail H.
AU - Wiktor, Ann
AU - Wise, Laura
AU - Yung, Jar Fee
AU - Zenger-Hain, Julie
AU - Zinsmeister, Alan
N1 - Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 1998/4/1
Y1 - 1998/4/1
N2 - Twenty-six laboratories used X and Y chromosome probes and the same procedures to process and examine 15,600 metaphases and 49,400 interphases from Phaseolus vulgaris-leucoagglutinin (PHA)-stimulated lymphocytes. In Part I, each laboratory scored 50 metaphases and 200 interphases from a normal male and a normal female from its own practice. In Part II, each laboratory scored 50 metaphases and 200 interphases on slides prepared by a central laboratory from a normal male and a normal female and three mixtures of cells from the male and female. In Part III, each laboratory scored 50 metaphases (in samples of 5, 10, 15, and 20) and 100 interphases (in samples of 5, 10, 15, 20, and 50) on new, coded slides of the same specimens used in Part II. Metaphases from male specimens were scored as 98-99% XY with no XX cells, and 97-98% of interphases were scored as XY with 0.04% XX cells. Metaphases from female specimens were scored as 96-97% XX with 0.03% XY cells, and 94-96% of interphases were scored as XX with 0.05% XY cells. Considering the data as a model for any probe used with fluorescence in situ hybridization (FISH), a statistical approach assessing the impact of analytical sensitivity on the numbers of observations required to assay for potential mosaicisms and chimerisms is discussed. The workload associated with processing slides and scoring 50 metaphases and 200 interphases using FISH averaged 27.1 and 28.6 minutes, respectively. This study indicates that multiple laboratories can test/develop guidelines for the rapid, efficacious, and cost-effective integration of FISH into clinical service.
AB - Twenty-six laboratories used X and Y chromosome probes and the same procedures to process and examine 15,600 metaphases and 49,400 interphases from Phaseolus vulgaris-leucoagglutinin (PHA)-stimulated lymphocytes. In Part I, each laboratory scored 50 metaphases and 200 interphases from a normal male and a normal female from its own practice. In Part II, each laboratory scored 50 metaphases and 200 interphases on slides prepared by a central laboratory from a normal male and a normal female and three mixtures of cells from the male and female. In Part III, each laboratory scored 50 metaphases (in samples of 5, 10, 15, and 20) and 100 interphases (in samples of 5, 10, 15, 20, and 50) on new, coded slides of the same specimens used in Part II. Metaphases from male specimens were scored as 98-99% XY with no XX cells, and 97-98% of interphases were scored as XY with 0.04% XX cells. Metaphases from female specimens were scored as 96-97% XX with 0.03% XY cells, and 94-96% of interphases were scored as XX with 0.05% XY cells. Considering the data as a model for any probe used with fluorescence in situ hybridization (FISH), a statistical approach assessing the impact of analytical sensitivity on the numbers of observations required to assay for potential mosaicisms and chimerisms is discussed. The workload associated with processing slides and scoring 50 metaphases and 200 interphases using FISH averaged 27.1 and 28.6 minutes, respectively. This study indicates that multiple laboratories can test/develop guidelines for the rapid, efficacious, and cost-effective integration of FISH into clinical service.
KW - Analytical sensitivity
KW - Chimerism
KW - Clone
KW - Interphase fluorescence in situ hybridization
KW - Mosaicism
KW - Quality assurance
KW - Workload
KW - X- and Y-chromosome probes
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U2 - 10.1002/(SICI)1096-8628(19980401)76:4<318::AID-AJMG7>3.0.CO;2-L
DO - 10.1002/(SICI)1096-8628(19980401)76:4<318::AID-AJMG7>3.0.CO;2-L
M3 - Article
C2 - 9545096
AN - SCOPUS:18344403136
SN - 0148-7299
VL - 76
SP - 318
EP - 326
JO - American journal of medical genetics
JF - American journal of medical genetics
IS - 4
ER -