A mouse model of familial oligoasthenoteratozoospermia

Subhash C. Juneja, Jan Van Deursen

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Background: Hrb is an HIV-1 Rev-binding/interacting protein and is a cofactor for Rev export pathway. Hrb interacts with Eps15 homology (EH) domain-containing proteins and is a component of EH network and functions in vesicle sorting. Earlier, we reported that Hrb-deficient male mice are infertile and that they show oligozoospermia. Their sperm lack acrosomes and present globozoospermia. The aim of this study was: (i) to investigate the additional defects in spermatogenesis in Hrb-deficient mice; and (ii) to investigate the effect of acrosomelessness on spermatid differentiation in Hrb-deficient mice. Methods: Hrb-/- testes, epididymides, spermatids and sperm were analyzed by histology and electron microscopy. Centrioles were analyzed in spermatids and sperm by indirect immunofluorescence technique. Results: Hrb-/- male mice exhibited multiple anomalies during meiosis and spermiogenesis that produced developmentally impaired sperm with unshaped or deformed nuclei, loss in cell polarity, intracellular flagellar coiling, multinucleation, supernumerary centrioles and multiflagellation. A total of 13.0% Hrb-/- sperm showed macrocephaly. The Hrb-/- sperm exhibited variation in head size and shape, disarranged cellular organelles, nuclear and cytoplasmic vacuolization, mitochondrial loss or scattering and no forward motility. Conclusions: These aberrations, in Hrb-/- mouse spermatids and sperm, are reminiscent of human familial male infertility with oligoasthenoteratozoospermia syndrome. The Hrb-deficient mouse may be useful in understanding familial oligoasthenoteratozoospermia syndrome.

Original languageEnglish (US)
Pages (from-to)881-893
Number of pages13
JournalHuman Reproduction
Volume20
Issue number4
DOIs
StatePublished - Apr 2005

Fingerprint

Oligospermia
Spermatozoa
Spermatids
Centrioles
Spermatogenesis
Megalencephaly
Acrosome
Cell Polarity
Epididymis
Male Infertility
Meiosis
Indirect Fluorescent Antibody Technique
Organelles
HIV-1
Testis
Histology
Electron Microscopy
Carrier Proteins
Head

Keywords

  • Centrioles
  • Manchette
  • Multiflagellation
  • Oligoasthenoteratozoospermia

ASJC Scopus subject areas

  • Developmental Biology
  • Physiology
  • Obstetrics and Gynecology
  • Reproductive Medicine

Cite this

A mouse model of familial oligoasthenoteratozoospermia. / Juneja, Subhash C.; Van Deursen, Jan.

In: Human Reproduction, Vol. 20, No. 4, 04.2005, p. 881-893.

Research output: Contribution to journalArticle

Juneja, Subhash C. ; Van Deursen, Jan. / A mouse model of familial oligoasthenoteratozoospermia. In: Human Reproduction. 2005 ; Vol. 20, No. 4. pp. 881-893.
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