A monoclonal antibody (MCPR3-7) interfering with the activity of proteinase 3 by an allosteric mechanism

Lisa C. Hinkofer, Susanne A I Seidel, Brice Korkmaz, Francisco Silva, Amber M. Hummel, Dieter Braun, Dieter E. Jenne, Ulrich Specks

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Background: Proteinase 3 is an abundant serine protease with high similarity to neutrophil elastase and a major autoimmune target in systemic vasculitis. Results: We identified a monoclonal antibody that inhibits PR3 activity. Conclusion: PR3-inhibiting antibodies can change its conformation and impair interactions with β1-proteinase inhibitor. Significance: PR3-inhibiting antibodies may play a role in autoimmune vasculitis and could be exploited as highly selective inhibitors.

Original languageEnglish (US)
Pages (from-to)26635-26648
Number of pages14
JournalJournal of Biological Chemistry
Volume288
Issue number37
DOIs
StatePublished - Sep 13 2013

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Myeloblastin
Monoclonal Antibodies
Systemic Vasculitis
Leukocyte Elastase
Antibodies
Serine Proteases
Vasculitis
Conformations
Peptide Hydrolases

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

A monoclonal antibody (MCPR3-7) interfering with the activity of proteinase 3 by an allosteric mechanism. / Hinkofer, Lisa C.; Seidel, Susanne A I; Korkmaz, Brice; Silva, Francisco; Hummel, Amber M.; Braun, Dieter; Jenne, Dieter E.; Specks, Ulrich.

In: Journal of Biological Chemistry, Vol. 288, No. 37, 13.09.2013, p. 26635-26648.

Research output: Contribution to journalArticle

Hinkofer, LC, Seidel, SAI, Korkmaz, B, Silva, F, Hummel, AM, Braun, D, Jenne, DE & Specks, U 2013, 'A monoclonal antibody (MCPR3-7) interfering with the activity of proteinase 3 by an allosteric mechanism', Journal of Biological Chemistry, vol. 288, no. 37, pp. 26635-26648. https://doi.org/10.1074/jbc.M113.495770
Hinkofer, Lisa C. ; Seidel, Susanne A I ; Korkmaz, Brice ; Silva, Francisco ; Hummel, Amber M. ; Braun, Dieter ; Jenne, Dieter E. ; Specks, Ulrich. / A monoclonal antibody (MCPR3-7) interfering with the activity of proteinase 3 by an allosteric mechanism. In: Journal of Biological Chemistry. 2013 ; Vol. 288, No. 37. pp. 26635-26648.
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