TY - JOUR
T1 - A molecular compendium of genes expressed in multiple myeloma
AU - Claudio, Jaime O.
AU - Masih-Khan, Esther
AU - Tang, Hongchang
AU - Gonçalves, Jason
AU - Voralia, Michael
AU - Li, Zhi Hua
AU - Nadeem, Vincent
AU - Cukerman, Eva
AU - Francisco-Pabalan, Ofelia
AU - Liew, Choong Chin
AU - Woodgett, James R.
AU - Keith Stewart, A.
PY - 2002/9/15
Y1 - 2002/9/15
N2 - We have created a molecular resource of genes expressed in primary malignant plasma cells using a combination of cDNA library construction, 5′ end single-pass sequencing, bioinformatics, and microarray analysis. In total, we identified 9732 nonredundant expressed genes. This data-set is available as the Myeloma Gene Index (www.uhnres.utoronto.ca/akstewart_lab). Predictably, the sequenced profile of myeloma cDNAs mirrored the known function of immunoglobulin-producing, high-respiratory rate, low-cycling, terminally differentiated plasma cells. Nevertheless, approximately 10% of myeloma-expressed sequences matched only entries in the database of Expressed Sequence Tags (dbEST) or the high-throughput genomic sequence (htgs) database. Numerous novel genes of potential biologic significance were identified. We therefore spotted 4300 sequenced cDNAs on glass slides creating a myeloma-enriched microarray. Several of the most highly expressed genes identified by sequencing, such as a novel putative disulfide isomerase (MGC3178), tumor rejection antigen TRA1, heat shock 70-kDa protein 5, and annexin A2, were also differentially expressed between myeloma and B lymphoma cell lines using this myeloma-enriched microarray. Furthermore, a defined subset of 34 up-regulated and 18 down-regulated genes on the array were able to differentiate myeloma from nonmyeloma cell lines. These not only include genes involved in B-cell biology such as syndecan, BCMA, PIM2, MUM1/IRF4, and XBP1, but also novel uncharacterized genes matching sequences only in the public databases. In summary, our expressed gene catalog and myeloma-enriched microarray contains numerous genes of unknown function and may complement other commercially available arrays in defining the molecular portrait of this hematopoietic malignancy.
AB - We have created a molecular resource of genes expressed in primary malignant plasma cells using a combination of cDNA library construction, 5′ end single-pass sequencing, bioinformatics, and microarray analysis. In total, we identified 9732 nonredundant expressed genes. This data-set is available as the Myeloma Gene Index (www.uhnres.utoronto.ca/akstewart_lab). Predictably, the sequenced profile of myeloma cDNAs mirrored the known function of immunoglobulin-producing, high-respiratory rate, low-cycling, terminally differentiated plasma cells. Nevertheless, approximately 10% of myeloma-expressed sequences matched only entries in the database of Expressed Sequence Tags (dbEST) or the high-throughput genomic sequence (htgs) database. Numerous novel genes of potential biologic significance were identified. We therefore spotted 4300 sequenced cDNAs on glass slides creating a myeloma-enriched microarray. Several of the most highly expressed genes identified by sequencing, such as a novel putative disulfide isomerase (MGC3178), tumor rejection antigen TRA1, heat shock 70-kDa protein 5, and annexin A2, were also differentially expressed between myeloma and B lymphoma cell lines using this myeloma-enriched microarray. Furthermore, a defined subset of 34 up-regulated and 18 down-regulated genes on the array were able to differentiate myeloma from nonmyeloma cell lines. These not only include genes involved in B-cell biology such as syndecan, BCMA, PIM2, MUM1/IRF4, and XBP1, but also novel uncharacterized genes matching sequences only in the public databases. In summary, our expressed gene catalog and myeloma-enriched microarray contains numerous genes of unknown function and may complement other commercially available arrays in defining the molecular portrait of this hematopoietic malignancy.
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U2 - 10.1182/blood-2002-01-0008
DO - 10.1182/blood-2002-01-0008
M3 - Article
C2 - 12200383
AN - SCOPUS:0037105582
SN - 0006-4971
VL - 100
SP - 2175
EP - 2186
JO - Blood
JF - Blood
IS - 6
ER -