A molecular compendium of genes expressed in multiple myeloma

Jaime O. Claudio, Esther Masih-Khan, Hongchang Tang, Jason Gonçalves, Michael Voralia, Zhi Hua Li, Vincent Nadeem, Eva Cukerman, Ofelia Francisco-Pabalan, Choong Chin Liew, James R. Woodgett, Alexander Keith Stewart

Research output: Contribution to journalArticle

135 Citations (Scopus)

Abstract

We have created a molecular resource of genes expressed in primary malignant plasma cells using a combination of cDNA library construction, 5′ end single-pass sequencing, bioinformatics, and microarray analysis. In total, we identified 9732 nonredundant expressed genes. This data-set is available as the Myeloma Gene Index (www.uhnres.utoronto.ca/akstewart_lab). Predictably, the sequenced profile of myeloma cDNAs mirrored the known function of immunoglobulin-producing, high-respiratory rate, low-cycling, terminally differentiated plasma cells. Nevertheless, approximately 10% of myeloma-expressed sequences matched only entries in the database of Expressed Sequence Tags (dbEST) or the high-throughput genomic sequence (htgs) database. Numerous novel genes of potential biologic significance were identified. We therefore spotted 4300 sequenced cDNAs on glass slides creating a myeloma-enriched microarray. Several of the most highly expressed genes identified by sequencing, such as a novel putative disulfide isomerase (MGC3178), tumor rejection antigen TRA1, heat shock 70-kDa protein 5, and annexin A2, were also differentially expressed between myeloma and B lymphoma cell lines using this myeloma-enriched microarray. Furthermore, a defined subset of 34 up-regulated and 18 down-regulated genes on the array were able to differentiate myeloma from nonmyeloma cell lines. These not only include genes involved in B-cell biology such as syndecan, BCMA, PIM2, MUM1/IRF4, and XBP1, but also novel uncharacterized genes matching sequences only in the public databases. In summary, our expressed gene catalog and myeloma-enriched microarray contains numerous genes of unknown function and may complement other commercially available arrays in defining the molecular portrait of this hematopoietic malignancy.

Original languageEnglish (US)
Pages (from-to)2175-2186
Number of pages12
JournalBlood
Volume100
Issue number6
DOIs
StatePublished - Sep 15 2002
Externally publishedYes

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Multiple Myeloma
Genes
Microarrays
Databases
Plasma Cells
Complementary DNA
Syndecans
Cells
Annexin A2
Cytology
Protein Disulfide-Isomerases
Plasmas
Cell Line
HSP70 Heat-Shock Proteins
Expressed Sequence Tags
Neoplasm Antigens
Hematologic Neoplasms
Microarray Analysis
Bioinformatics
Respiratory Rate

ASJC Scopus subject areas

  • Hematology

Cite this

Claudio, J. O., Masih-Khan, E., Tang, H., Gonçalves, J., Voralia, M., Li, Z. H., ... Stewart, A. K. (2002). A molecular compendium of genes expressed in multiple myeloma. Blood, 100(6), 2175-2186. https://doi.org/10.1182/blood-2002-01-0008

A molecular compendium of genes expressed in multiple myeloma. / Claudio, Jaime O.; Masih-Khan, Esther; Tang, Hongchang; Gonçalves, Jason; Voralia, Michael; Li, Zhi Hua; Nadeem, Vincent; Cukerman, Eva; Francisco-Pabalan, Ofelia; Liew, Choong Chin; Woodgett, James R.; Stewart, Alexander Keith.

In: Blood, Vol. 100, No. 6, 15.09.2002, p. 2175-2186.

Research output: Contribution to journalArticle

Claudio, JO, Masih-Khan, E, Tang, H, Gonçalves, J, Voralia, M, Li, ZH, Nadeem, V, Cukerman, E, Francisco-Pabalan, O, Liew, CC, Woodgett, JR & Stewart, AK 2002, 'A molecular compendium of genes expressed in multiple myeloma', Blood, vol. 100, no. 6, pp. 2175-2186. https://doi.org/10.1182/blood-2002-01-0008
Claudio JO, Masih-Khan E, Tang H, Gonçalves J, Voralia M, Li ZH et al. A molecular compendium of genes expressed in multiple myeloma. Blood. 2002 Sep 15;100(6):2175-2186. https://doi.org/10.1182/blood-2002-01-0008
Claudio, Jaime O. ; Masih-Khan, Esther ; Tang, Hongchang ; Gonçalves, Jason ; Voralia, Michael ; Li, Zhi Hua ; Nadeem, Vincent ; Cukerman, Eva ; Francisco-Pabalan, Ofelia ; Liew, Choong Chin ; Woodgett, James R. ; Stewart, Alexander Keith. / A molecular compendium of genes expressed in multiple myeloma. In: Blood. 2002 ; Vol. 100, No. 6. pp. 2175-2186.
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AU - Nadeem, Vincent

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