TY - JOUR
T1 - A model of food-borne Listeria monocytogenes infection in the Sprague-Dawley rat using gastric inoculation
T2 - development and effect of gastric acidity on infective dose
AU - Schlech, Walter F.
AU - Chase, Daniel P.
AU - Badley, Andrew
N1 - Funding Information:
This work was supported in part by a scholarship to Dr Schlech from the Canadian Life and Health Insurance Association, an operating grant from the University Faculty of Medicine, and grants from the Wisconsin Milk Marketing Board and the International Life Sciences Nutrition Foundation. The authors would like to thank Peter Nicholson and Dr Gary Faulkner for electronmicro-scopic studies and the Department of Pathology, Victoria General Hospital for performing histologic studies.
PY - 1993/3
Y1 - 1993/3
N2 - Recent epidemiological evidence suggests that Listeria monocytogenes (LM) is a food-borne pathogen in humans. A model of LM infection was developed using the Sprague-Dawley (SD) rat to study the interaction of LM with gastrointestinal epithelium as the first step in the pathogenesis of invasive listeriosis. Conventionally raised, juvenile female SD rats were given 102-109 virulent L. monocytogenes, serotype 4b or nonpathogenic Listeria species. Only rats given virulent LM developed dose-dependent invasive infection of the liver and spleen. Light and electron microscopic studies suggested attachment to and invasion of the gastrointestinal mucosa by virulent LM. Because the development of invasive listeriosis in humans has been epidemiologically associated with a decrease in gastric acidity, the effect of decreasing gastric acidity on dose-dependent infection was studied. Rats were pretreated with cimetidine (50 mg/kg) by intraperitoneal injection prior to oral inoculation of 102-109 virulent L. monocytogenes. Cimetidine significantly lowered the infective dose of virulent L. monocytogenes (P<0.05). This oral model should allow further study of host and organism-specific virulence factors mediating the gastrointestinal phase of invasive LM infection, an increasingly important public health problem.
AB - Recent epidemiological evidence suggests that Listeria monocytogenes (LM) is a food-borne pathogen in humans. A model of LM infection was developed using the Sprague-Dawley (SD) rat to study the interaction of LM with gastrointestinal epithelium as the first step in the pathogenesis of invasive listeriosis. Conventionally raised, juvenile female SD rats were given 102-109 virulent L. monocytogenes, serotype 4b or nonpathogenic Listeria species. Only rats given virulent LM developed dose-dependent invasive infection of the liver and spleen. Light and electron microscopic studies suggested attachment to and invasion of the gastrointestinal mucosa by virulent LM. Because the development of invasive listeriosis in humans has been epidemiologically associated with a decrease in gastric acidity, the effect of decreasing gastric acidity on dose-dependent infection was studied. Rats were pretreated with cimetidine (50 mg/kg) by intraperitoneal injection prior to oral inoculation of 102-109 virulent L. monocytogenes. Cimetidine significantly lowered the infective dose of virulent L. monocytogenes (P<0.05). This oral model should allow further study of host and organism-specific virulence factors mediating the gastrointestinal phase of invasive LM infection, an increasingly important public health problem.
KW - Gastric acidity
KW - Gastrointestinal mucosa
KW - Invasion
KW - Listeria monocytogenes
KW - Virulence
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U2 - 10.1016/0168-1605(93)90003-Y
DO - 10.1016/0168-1605(93)90003-Y
M3 - Article
C2 - 8466809
AN - SCOPUS:0027468453
SN - 0168-1605
VL - 18
SP - 15
EP - 24
JO - International Journal of Food Microbiology
JF - International Journal of Food Microbiology
IS - 1
ER -