A microRNA biomarker panel for the non-invasive detection of bladder cancer

Virginia Urquidi, Mandy Netherton, Evan Gomes-Giacoia, Daniel J. Serie, Jeanette E Eckel-Passow, Charles J. Rosser, Steven Goodison

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The development of accurate, non-invasive urinary assays for bladder cancer would greatly facilitate the detection and management of a disease that has a high rate of recurrence and progression. In this study, we employed a discovery and validation strategy to identify microRNA signatures that can perform as a non-invasive bladder cancer diagnostic assay. Expression profiling of 754 human microRNAs (TaqMan low density arrays) was performed on naturally voided urine samples from a cohort of 85 subjects of known bladder disease status (27 with active BCa). A panel of 46 microRNAs significantly associated with bladder cancer were subsequently monitored in an independent cohort of 121 subjects (61 with active BCa) using quantitative real-time PCR (RT-PCR). Multivariable modeling identified a 25-target diagnostic signature that predicted the presence of BCa with an estimated sensitivity of 87% at a specificity of 100% (AUC 0.982). With additional validation, the monitoring of a urinary microRNA biomarker panel could facilitate the non-invasive evaluation of patients under investigation for BCa.

Original languageEnglish (US)
Pages (from-to)86290-86299
Number of pages10
JournalOncotarget
Volume7
Issue number52
StatePublished - 2016

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MicroRNAs
Urinary Bladder Neoplasms
Biomarkers
Urinary Bladder Diseases
Disease Management
Area Under Curve
Real-Time Polymerase Chain Reaction
Urine
Recurrence

Keywords

  • Bladder cancer
  • Diagnostic biomarkers
  • MicroRNA
  • Multiplex
  • Urinalysis

ASJC Scopus subject areas

  • Oncology

Cite this

Urquidi, V., Netherton, M., Gomes-Giacoia, E., Serie, D. J., Eckel-Passow, J. E., Rosser, C. J., & Goodison, S. (2016). A microRNA biomarker panel for the non-invasive detection of bladder cancer. Oncotarget, 7(52), 86290-86299.

A microRNA biomarker panel for the non-invasive detection of bladder cancer. / Urquidi, Virginia; Netherton, Mandy; Gomes-Giacoia, Evan; Serie, Daniel J.; Eckel-Passow, Jeanette E; Rosser, Charles J.; Goodison, Steven.

In: Oncotarget, Vol. 7, No. 52, 2016, p. 86290-86299.

Research output: Contribution to journalArticle

Urquidi, V, Netherton, M, Gomes-Giacoia, E, Serie, DJ, Eckel-Passow, JE, Rosser, CJ & Goodison, S 2016, 'A microRNA biomarker panel for the non-invasive detection of bladder cancer', Oncotarget, vol. 7, no. 52, pp. 86290-86299.
Urquidi V, Netherton M, Gomes-Giacoia E, Serie DJ, Eckel-Passow JE, Rosser CJ et al. A microRNA biomarker panel for the non-invasive detection of bladder cancer. Oncotarget. 2016;7(52):86290-86299.
Urquidi, Virginia ; Netherton, Mandy ; Gomes-Giacoia, Evan ; Serie, Daniel J. ; Eckel-Passow, Jeanette E ; Rosser, Charles J. ; Goodison, Steven. / A microRNA biomarker panel for the non-invasive detection of bladder cancer. In: Oncotarget. 2016 ; Vol. 7, No. 52. pp. 86290-86299.
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