A microRNA-328 binding site in PAX6 is associated with centrotemporal spikes of rolandic epilepsy

Naim Panjwani, Michael D. Wilson, Laura Addis, Jennifer Crosbie, Elaine C Wirrell, Stéphane Auvin, Roberto H. Caraballo, Maria Kinali, David McCormick, Caroline Oren, Jacqueline Taylor, John Trounce, Tara Clarke, Cigdem I. Akman, Steven L. Kugler, David E. Mandelbaum, Patricia McGoldrick, Steven M. Wolf, Paul Arnold, Russell SchacharDeb K. Pal, Lisa J. Strug

Research output: Contribution to journalArticle

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Abstract

Objective: Rolandic epilepsy is a common genetic focal epilepsy of childhood characterized by centrotemporal sharp waves on electroencephalogram. In previous genome-wide analysis, we had reported linkage of centrotemporal sharp waves to chromosome 11p13, and fine mapping with 44 SNPs identified the ELP4-PAX6 locus in two independent US and Canadian case–control samples. Here, we aimed to find a causative variant for centrotemporal sharp waves using a larger sample and higher resolution genotyping array. Methods: We fine-mapped the ELP4-PAX6 locus in 186 individuals from rolandic epilepsy families and 1000 population controls of European origin using the Illumina HumanCoreExome-12 v1.0 BeadChip. Controls were matched to cases on ethnicity using principal component analysis. We used generalized estimating equations to assess association, followed up with a bioinformatics survey and literature search to evaluate functional significance. Results: Homozygosity at the T allele of SNP rs662702 in the 3′ untranslated region of PAX6 conferred increased risk of CTS: Odds ratio = 12.29 (95% CI: 3.20–47.22), P = 2.6 × 10−4 and is seen in 3.9% of cases but only 0.3% of controls. Interpretation: The minor T allele of SNP rs662702 disrupts regulation by microRNA-328, which is known to result in increased PAX6 expression in vitro. This study provides, for the first time, evidence of a noncoding genomic variant contributing to the etiology of a common human epilepsy via a posttranscriptional regulatory mechanism.

Original languageEnglish (US)
Pages (from-to)512-522
Number of pages11
JournalAnnals of Clinical and Translational Neurology
DOIs
StatePublished - Jul 1 2016

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Rolandic Epilepsy
MicroRNAs
Single Nucleotide Polymorphism
Binding Sites
Alleles
Population Control
Partial Epilepsy
3' Untranslated Regions
Principal Component Analysis
Computational Biology
Electroencephalography
Epilepsy
Chromosomes
Odds Ratio
Genome

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology

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A microRNA-328 binding site in PAX6 is associated with centrotemporal spikes of rolandic epilepsy. / Panjwani, Naim; Wilson, Michael D.; Addis, Laura; Crosbie, Jennifer; Wirrell, Elaine C; Auvin, Stéphane; Caraballo, Roberto H.; Kinali, Maria; McCormick, David; Oren, Caroline; Taylor, Jacqueline; Trounce, John; Clarke, Tara; Akman, Cigdem I.; Kugler, Steven L.; Mandelbaum, David E.; McGoldrick, Patricia; Wolf, Steven M.; Arnold, Paul; Schachar, Russell; Pal, Deb K.; Strug, Lisa J.

In: Annals of Clinical and Translational Neurology, 01.07.2016, p. 512-522.

Research output: Contribution to journalArticle

Panjwani, N, Wilson, MD, Addis, L, Crosbie, J, Wirrell, EC, Auvin, S, Caraballo, RH, Kinali, M, McCormick, D, Oren, C, Taylor, J, Trounce, J, Clarke, T, Akman, CI, Kugler, SL, Mandelbaum, DE, McGoldrick, P, Wolf, SM, Arnold, P, Schachar, R, Pal, DK & Strug, LJ 2016, 'A microRNA-328 binding site in PAX6 is associated with centrotemporal spikes of rolandic epilepsy', Annals of Clinical and Translational Neurology, pp. 512-522. https://doi.org/10.1002/acn3.320
Panjwani, Naim ; Wilson, Michael D. ; Addis, Laura ; Crosbie, Jennifer ; Wirrell, Elaine C ; Auvin, Stéphane ; Caraballo, Roberto H. ; Kinali, Maria ; McCormick, David ; Oren, Caroline ; Taylor, Jacqueline ; Trounce, John ; Clarke, Tara ; Akman, Cigdem I. ; Kugler, Steven L. ; Mandelbaum, David E. ; McGoldrick, Patricia ; Wolf, Steven M. ; Arnold, Paul ; Schachar, Russell ; Pal, Deb K. ; Strug, Lisa J. / A microRNA-328 binding site in PAX6 is associated with centrotemporal spikes of rolandic epilepsy. In: Annals of Clinical and Translational Neurology. 2016 ; pp. 512-522.
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abstract = "Objective: Rolandic epilepsy is a common genetic focal epilepsy of childhood characterized by centrotemporal sharp waves on electroencephalogram. In previous genome-wide analysis, we had reported linkage of centrotemporal sharp waves to chromosome 11p13, and fine mapping with 44 SNPs identified the ELP4-PAX6 locus in two independent US and Canadian case–control samples. Here, we aimed to find a causative variant for centrotemporal sharp waves using a larger sample and higher resolution genotyping array. Methods: We fine-mapped the ELP4-PAX6 locus in 186 individuals from rolandic epilepsy families and 1000 population controls of European origin using the Illumina HumanCoreExome-12 v1.0 BeadChip. Controls were matched to cases on ethnicity using principal component analysis. We used generalized estimating equations to assess association, followed up with a bioinformatics survey and literature search to evaluate functional significance. Results: Homozygosity at the T allele of SNP rs662702 in the 3′ untranslated region of PAX6 conferred increased risk of CTS: Odds ratio = 12.29 (95{\%} CI: 3.20–47.22), P = 2.6 × 10−4 and is seen in 3.9{\%} of cases but only 0.3{\%} of controls. Interpretation: The minor T allele of SNP rs662702 disrupts regulation by microRNA-328, which is known to result in increased PAX6 expression in vitro. This study provides, for the first time, evidence of a noncoding genomic variant contributing to the etiology of a common human epilepsy via a posttranscriptional regulatory mechanism.",
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T1 - A microRNA-328 binding site in PAX6 is associated with centrotemporal spikes of rolandic epilepsy

AU - Panjwani, Naim

AU - Wilson, Michael D.

AU - Addis, Laura

AU - Crosbie, Jennifer

AU - Wirrell, Elaine C

AU - Auvin, Stéphane

AU - Caraballo, Roberto H.

AU - Kinali, Maria

AU - McCormick, David

AU - Oren, Caroline

AU - Taylor, Jacqueline

AU - Trounce, John

AU - Clarke, Tara

AU - Akman, Cigdem I.

AU - Kugler, Steven L.

AU - Mandelbaum, David E.

AU - McGoldrick, Patricia

AU - Wolf, Steven M.

AU - Arnold, Paul

AU - Schachar, Russell

AU - Pal, Deb K.

AU - Strug, Lisa J.

PY - 2016/7/1

Y1 - 2016/7/1

N2 - Objective: Rolandic epilepsy is a common genetic focal epilepsy of childhood characterized by centrotemporal sharp waves on electroencephalogram. In previous genome-wide analysis, we had reported linkage of centrotemporal sharp waves to chromosome 11p13, and fine mapping with 44 SNPs identified the ELP4-PAX6 locus in two independent US and Canadian case–control samples. Here, we aimed to find a causative variant for centrotemporal sharp waves using a larger sample and higher resolution genotyping array. Methods: We fine-mapped the ELP4-PAX6 locus in 186 individuals from rolandic epilepsy families and 1000 population controls of European origin using the Illumina HumanCoreExome-12 v1.0 BeadChip. Controls were matched to cases on ethnicity using principal component analysis. We used generalized estimating equations to assess association, followed up with a bioinformatics survey and literature search to evaluate functional significance. Results: Homozygosity at the T allele of SNP rs662702 in the 3′ untranslated region of PAX6 conferred increased risk of CTS: Odds ratio = 12.29 (95% CI: 3.20–47.22), P = 2.6 × 10−4 and is seen in 3.9% of cases but only 0.3% of controls. Interpretation: The minor T allele of SNP rs662702 disrupts regulation by microRNA-328, which is known to result in increased PAX6 expression in vitro. This study provides, for the first time, evidence of a noncoding genomic variant contributing to the etiology of a common human epilepsy via a posttranscriptional regulatory mechanism.

AB - Objective: Rolandic epilepsy is a common genetic focal epilepsy of childhood characterized by centrotemporal sharp waves on electroencephalogram. In previous genome-wide analysis, we had reported linkage of centrotemporal sharp waves to chromosome 11p13, and fine mapping with 44 SNPs identified the ELP4-PAX6 locus in two independent US and Canadian case–control samples. Here, we aimed to find a causative variant for centrotemporal sharp waves using a larger sample and higher resolution genotyping array. Methods: We fine-mapped the ELP4-PAX6 locus in 186 individuals from rolandic epilepsy families and 1000 population controls of European origin using the Illumina HumanCoreExome-12 v1.0 BeadChip. Controls were matched to cases on ethnicity using principal component analysis. We used generalized estimating equations to assess association, followed up with a bioinformatics survey and literature search to evaluate functional significance. Results: Homozygosity at the T allele of SNP rs662702 in the 3′ untranslated region of PAX6 conferred increased risk of CTS: Odds ratio = 12.29 (95% CI: 3.20–47.22), P = 2.6 × 10−4 and is seen in 3.9% of cases but only 0.3% of controls. Interpretation: The minor T allele of SNP rs662702 disrupts regulation by microRNA-328, which is known to result in increased PAX6 expression in vitro. This study provides, for the first time, evidence of a noncoding genomic variant contributing to the etiology of a common human epilepsy via a posttranscriptional regulatory mechanism.

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