TY - GEN
T1 - A Microfluidic System Combining Valve Automation and Spheroid Cultures to Characterize Hepatic Glucose Metabolism during Hormonal Stimulation
AU - Cedillo-Alcantar, DIana F.
AU - Stybayeva, Gulnaz
AU - De Hoyos-Vega, Jose M.
AU - Duk Han, Yong
AU - Choi, Jonghoon
AU - Garcia-Cordero, Jose L.
AU - Revzin, Alexander
PY - 2019/6/1
Y1 - 2019/6/1
N2 - We describe an automated microsystem integrating hepatocyte spheroid culture and droplet-based bioanalysis modules. This device was shown to be suitable for long-term maintenance of primary hepatocytes. Importantly, this device was outfitted with microvalves to modulate composition of the input media with high temporal control. This ability was used to stimulate hepatocytes with waives of hormones, insulin and glucagon, so as to mimic physiological composition of hormones in the hepatic portal circulation. Using on-chip bioanalysis module we demonstrate that hepatocytes behave in physiological manner, consuming glucose upon stimulation with insulin and releasing glucose upon exposure to glucagon.
AB - We describe an automated microsystem integrating hepatocyte spheroid culture and droplet-based bioanalysis modules. This device was shown to be suitable for long-term maintenance of primary hepatocytes. Importantly, this device was outfitted with microvalves to modulate composition of the input media with high temporal control. This ability was used to stimulate hepatocytes with waives of hormones, insulin and glucagon, so as to mimic physiological composition of hormones in the hepatic portal circulation. Using on-chip bioanalysis module we demonstrate that hepatocytes behave in physiological manner, consuming glucose upon stimulation with insulin and releasing glucose upon exposure to glucagon.
KW - Droplet-microfluidics
KW - Glucose homeostasis
KW - Hormonal stimulation
KW - Liver-on-a-chip
KW - Multiplexed biosensing
KW - Real-time tissue monitoring
UR - http://www.scopus.com/inward/record.url?scp=85071953248&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85071953248&partnerID=8YFLogxK
U2 - 10.1109/TRANSDUCERS.2019.8808554
DO - 10.1109/TRANSDUCERS.2019.8808554
M3 - Conference contribution
T3 - 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII
SP - 488
EP - 491
BT - 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII
Y2 - 23 June 2019 through 27 June 2019
ER -