A Microfluidic System Combining Valve Automation and Spheroid Cultures to Characterize Hepatic Glucose Metabolism during Hormonal Stimulation

DIana F. Cedillo-Alcantar, Gulnaz Stybayeva, Jose M. De Hoyos-Vega, Yong Duk Han, Jonghoon Choi, Jose L. Garcia-Cordero, Alexander Revzin

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We describe an automated microsystem integrating hepatocyte spheroid culture and droplet-based bioanalysis modules. This device was shown to be suitable for long-term maintenance of primary hepatocytes. Importantly, this device was outfitted with microvalves to modulate composition of the input media with high temporal control. This ability was used to stimulate hepatocytes with waives of hormones, insulin and glucagon, so as to mimic physiological composition of hormones in the hepatic portal circulation. Using on-chip bioanalysis module we demonstrate that hepatocytes behave in physiological manner, consuming glucose upon stimulation with insulin and releasing glucose upon exposure to glucagon.

Original languageEnglish (US)
Title of host publication2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages488-491
Number of pages4
ISBN (Electronic)9781728120072
DOIs
StatePublished - Jun 1 2019
Event20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII - Berlin, Germany
Duration: Jun 23 2019Jun 27 2019

Publication series

Name2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII

Conference

Conference20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII
CountryGermany
CityBerlin
Period6/23/196/27/19

Keywords

  • Droplet-microfluidics
  • Glucose homeostasis
  • Hormonal stimulation
  • Liver-on-a-chip
  • Multiplexed biosensing
  • Real-time tissue monitoring

ASJC Scopus subject areas

  • Process Chemistry and Technology
  • Spectroscopy
  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Electronic, Optical and Magnetic Materials
  • Control and Optimization
  • Instrumentation

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  • Cite this

    Cedillo-Alcantar, DI. F., Stybayeva, G., De Hoyos-Vega, J. M., Duk Han, Y., Choi, J., Garcia-Cordero, J. L., & Revzin, A. (2019). A Microfluidic System Combining Valve Automation and Spheroid Cultures to Characterize Hepatic Glucose Metabolism during Hormonal Stimulation. In 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII (pp. 488-491). [8808554] (2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/TRANSDUCERS.2019.8808554