A microfabricated device for the characterization of biological entities

H. Chang, A. Ikram, M. Young, F. Kosari, G. Vasmatzis, A. Bhunia, R. Bashir

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A micro-fabricated pore is constructed and tested so that it can be used to characterize biological entities. The pore is prepared by bulk micro-machining of a silicon wafer. An oxide coated silicon diaphragm with the pore is placed between two chambers containing ionic buffer solutions to mimic a bilayer system. If a voltage is applied across the pore, electrophorelic passage of charged entities can be electrically detected through changes in the ionic current flow. When the entities traverse the pore, the ionic current is blocked and a decrease in the current can be observed. As an initial test case, negatively charged polystyrene beads which were 2.38nm in diameter, were electrophoretically driven across the pore. Then the bacterium Listeria innocua, suspended in Tris-glycine buffer, was also electrophoretically driven through the pore and its effective mobility was extracted. The device can also be used to study the interactions between organisms and the micro-fabricated surfaces. Work is continuing to scale the pore to the sub100A range to be used for characterization and possible sequencing of single molecules such as DNA.

Original languageEnglish (US)
Title of host publicationMolecular and Biomolecular Electronics
PublisherMaterials Research Society
Pages43-48
Number of pages6
ISBN (Print)155899615X, 9781558996151
DOIs
StatePublished - Jan 1 2001
Event2001 MRS Spring Meeting - San Francisco, CA, United States
Duration: Apr 16 2001Apr 20 2001

Publication series

NameMaterials Research Society Symposium Proceedings
Volume679
ISSN (Print)0272-9172

Other

Other2001 MRS Spring Meeting
CountryUnited States
CitySan Francisco, CA
Period4/16/014/20/01

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

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  • Cite this

    Chang, H., Ikram, A., Young, M., Kosari, F., Vasmatzis, G., Bhunia, A., & Bashir, R. (2001). A microfabricated device for the characterization of biological entities. In Molecular and Biomolecular Electronics (pp. 43-48). (Materials Research Society Symposium Proceedings; Vol. 679). Materials Research Society. https://doi.org/10.1557/proc-679-b3.3