A mecA-negative strain of methicillin-resistant Staphylococcus aureus with high-level β-lactam resistance contains mutations in three genes

Ritu Banerjee; Michael Gretes; Christopher Harlem; Li Basuino; Henry F. Chambers

doi:10.1128/AAC.00594-10

A mecA-negative strain of methicillin-resistant Staphylococcus aureus with high-level β-lactam resistance contains mutations in three genes

Ritu Banerjee, Michael Gretes, Christopher Harlem, Li Basuino, Henry F. Chambers

Pediatric and Adolescent Medicine

Research output: Contribution to journal › Article › peer-review

62 Scopus citations

Abstract

We previously generated a ceftobiprole-resistant Staphylococcus aureus strain after high inoculum serial passage of a mecA-negative variant of strain COL (R. Banerjee, M. Gretes, L. Basuino, N. Strynadka, and H. F. Chambers, Antimicrob. Agents Chemother. 52:2089-2096, 2008). Genome resequencing of this strain, CRB, revealed that it differs from its parent by five single-nucleotide polymorphisms in three genes, specifically, those encoding PBP4, a low-molecular-weight penicillin-binding protein, GdpP, a predicted signaling protein, and AcrB, a cation multidrug efflux transporter. CRB displayed resistance to a variety of β-lactams but was hypersusceptible to cefoxitin.

Original language	English (US)
Pages (from-to)	4900-4902
Number of pages	3
Journal	Antimicrobial Agents and Chemotherapy
Volume	54
Issue number	11
DOIs	https://doi.org/10.1128/AAC.00594-10
State	Published - Nov 2010

ASJC Scopus subject areas

Pharmacology
Pharmacology (medical)
Infectious Diseases

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abstract = "We previously generated a ceftobiprole-resistant Staphylococcus aureus strain after high inoculum serial passage of a mecA-negative variant of strain COL (R. Banerjee, M. Gretes, L. Basuino, N. Strynadka, and H. F. Chambers, Antimicrob. Agents Chemother. 52:2089-2096, 2008). Genome resequencing of this strain, CRB, revealed that it differs from its parent by five single-nucleotide polymorphisms in three genes, specifically, those encoding PBP4, a low-molecular-weight penicillin-binding protein, GdpP, a predicted signaling protein, and AcrB, a cation multidrug efflux transporter. CRB displayed resistance to a variety of β-lactams but was hypersusceptible to cefoxitin.",

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AU - Gretes, Michael

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AU - Basuino, Li

AU - Chambers, Henry F.

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A mecA-negative strain of methicillin-resistant Staphylococcus aureus with high-level β-lactam resistance contains mutations in three genes

Abstract

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