A mass spectrometric approach for characterization of amyloid-β aggregates and identification of their post-translational modifications

William M. Tay, Jennifer G. Bryant, Patricia K. Martin, A. Jeremy Nix, Bernadette M. Cusack, Terrone L. Rosenberry

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Endogenous amyloid-β (Aβ) oligomeric aggregates have been proposed as toxic agents in Alzheimer's disease (AD). Knowledge of their structures not only may provide insight into the basis of their neurotoxicities but also may reveal new targets for therapeutic drugs and diagnostic tools. However, the low levels of these Aβ oligomers have impeded structural characterization. Evidence suggests that the endogenous oligomers are covalently modified in vivo. In this report, we demonstrate an established mass spectrometry (MS) methodology called precursor ion mapping (PIM) that potentially may be applied to endogenous oligomer characterization. First, we illustrate the use of this PIM technique with a synthetic Aβ(1-40) monomer sample that had been cross-linked with transglutaminase (TGase) and digested with pepsin. From PIM analysis of an Aβ(4-13) MS/MS fragment, precursor ions were identified that corresponded to peptic fragments of three TGase cross-linked species: Aβ(4-19) - (4-19), Aβ(4-19) - (20-34), and Aβ(1-19) - (20-34). Next, we demonstrate the applicability of the PIM technique to an endogenous Aβ sample that had been purified and concentrated by immunoaffinity chromatography. Without pepsin digestion, we successfully identified the full length and C-terminally truncated monomeric Aβ species 1-35 to 1-42, along with select methionine-oxidized counterparts. Because PIM focuses only on a subpopulation of ions, namely the related precursor ions, the resulting spectra are of increased specificity and sensitivity. Therefore, this methodology shows great promise for structural analysis and identification of post-translational modification(s) in endogenous Aβ oligomers.

Original languageEnglish (US)
Pages (from-to)3759-3766
Number of pages8
JournalBiochemistry
Volume51
Issue number18
DOIs
StatePublished - May 8 2012

ASJC Scopus subject areas

  • Biochemistry

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