A low molecular weight glycoprotein associated with isolated myelin: distinction from myelin proteolipid protein

J. F. Poduslo, J. L. Everly, R. H. Quarles

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

A low mol wt glycoprotein has been demonstrated in myelin isolated from immature rat brains. Both short term and long term fucose incorporation studies have identified this protein in the proteolipid protein region of a sodium dodecyl sulfate, polyacrylamide gal. A 1.7 to 2.1 fold increase in radioactive fucose in this glycoprotein relative to the major myelin glycoprotein was seen after long term fucose incorporation (21 days) compared to short term incorporation (18 to 22 hr). The demonstration that this fucose labelled protein is distinguishable from that of proteolipid protein was achieved by a variety of independent techniques. 1 Technique involved a comparison of either ethanol extracted, freshly isolated, myelin with myelin extracted with chloroform methanol. Treatment of isolated myelin with chloroform methanol results in the solubilization of the proteolipid protein and its subsequent absence on gel electrophoresis while, in contrast, an enhancement of fucose label was observed in the same region of the polyacrylamide gel. Another procedure involved the electrophoretic separation of the radioactive fucose peak from that of proteolipid protein by employing a continuous, phosphate buffered, gel system. Finally carbohydrate analysis by gas liquid chromatography of a partially purified proteolipid protein fraction did not reveal significant amounts of carbohydrates which are characteristic of glycoproteins. The identification of this minor glycoprotein comigrating with proteolipid proteins indicates, therefore, a greater complexity associated with the purified myelin membrane than has been previously demonstrated.

Original languageEnglish (US)
Pages (from-to)977-986
Number of pages10
JournalJournal of Neurochemistry
Volume28
Issue number5
StatePublished - 1977
Externally publishedYes

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Myelin Proteolipid Protein
Myelin Sheath
Proteolipids
Fucose
Glycoproteins
Molecular Weight
Molecular weight
Proteins
Chloroform
Methanol
Gels
Carbohydrates
Liquid chromatography
Electrophoresis
Sodium Dodecyl Sulfate
Gas chromatography
Gas Chromatography
Rats
Labels
Brain

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

A low molecular weight glycoprotein associated with isolated myelin : distinction from myelin proteolipid protein. / Poduslo, J. F.; Everly, J. L.; Quarles, R. H.

In: Journal of Neurochemistry, Vol. 28, No. 5, 1977, p. 977-986.

Research output: Contribution to journalArticle

Poduslo, J. F. ; Everly, J. L. ; Quarles, R. H. / A low molecular weight glycoprotein associated with isolated myelin : distinction from myelin proteolipid protein. In: Journal of Neurochemistry. 1977 ; Vol. 28, No. 5. pp. 977-986.
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