A low dose of ionizing radiation increases luminal release of intestinal peptidases in rats

Marina Fittkau, Axel F Grothey, Reinhard Gerlach, Hans Joachim Schmoll

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Purpose: Mucosal inflammation in the small intestine is a potentially hazardous side effect of abdominal irradiation. In an effort to develop a quantitative method of evaluating mucosal damage, the luminal release of brush border enzymes in response to ionizing radiation was examined using two investigational strategies. Methods: First, a 20 cm segment of the proximal jejunum was perfused in situ and enzymatic activities within the perfusates were evaluated. In a second approach, enzymatic activities were directly evaluated in isolated brush border membranes from the jejunal mucosa. Results: Most of the peptidase activities measured were increased in the perfusates 1 day after irradiation and had returned to control levels at 4 days. In the brush border membranes, some enzyme activities decreased at 1 day and were, with the exception of leucineaminopeptidase (LAP), similar to control levels at 4 days. Conclusions: LAP is more strongly affected by radiation than the transmembranously bounded enzymes.

Original languageEnglish (US)
Pages (from-to)96-100
Number of pages5
JournalJournal of Cancer Research and Clinical Oncology
Volume127
Issue number2
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Microvilli
Ionizing Radiation
Peptide Hydrolases
Enzymes
Membranes
Jejunum
Small Intestine
Mucous Membrane
Radiation
Inflammation

Keywords

  • Brush border membrane
  • Intestinal enzymes
  • Perfusion
  • Radiation
  • Rats

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

A low dose of ionizing radiation increases luminal release of intestinal peptidases in rats. / Fittkau, Marina; Grothey, Axel F; Gerlach, Reinhard; Schmoll, Hans Joachim.

In: Journal of Cancer Research and Clinical Oncology, Vol. 127, No. 2, 2001, p. 96-100.

Research output: Contribution to journalArticle

Fittkau, Marina ; Grothey, Axel F ; Gerlach, Reinhard ; Schmoll, Hans Joachim. / A low dose of ionizing radiation increases luminal release of intestinal peptidases in rats. In: Journal of Cancer Research and Clinical Oncology. 2001 ; Vol. 127, No. 2. pp. 96-100.
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