Abstract
Purpose: Mucosal inflammation in the small intestine is a potentially hazardous side effect of abdominal irradiation. In an effort to develop a quantitative method of evaluating mucosal damage, the luminal release of brush border enzymes in response to ionizing radiation was examined using two investigational strategies. Methods: First, a 20 cm segment of the proximal jejunum was perfused in situ and enzymatic activities within the perfusates were evaluated. In a second approach, enzymatic activities were directly evaluated in isolated brush border membranes from the jejunal mucosa. Results: Most of the peptidase activities measured were increased in the perfusates 1 day after irradiation and had returned to control levels at 4 days. In the brush border membranes, some enzyme activities decreased at 1 day and were, with the exception of leucineaminopeptidase (LAP), similar to control levels at 4 days. Conclusions: LAP is more strongly affected by radiation than the transmembranously bounded enzymes.
Original language | English (US) |
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Pages (from-to) | 96-100 |
Number of pages | 5 |
Journal | Journal of Cancer Research and Clinical Oncology |
Volume | 127 |
Issue number | 2 |
DOIs | |
State | Published - 2001 |
Keywords
- Brush border membrane
- Intestinal enzymes
- Perfusion
- Radiation
- Rats
ASJC Scopus subject areas
- Oncology
- Cancer Research