TY - JOUR
T1 - A long-range restriction map between the α-globin complex and a marker closely linked to the polycystic kidney disease 1 (PKD1) locus
AU - Harris, P. C.
AU - Barton, N. J.
AU - Higgs, D. R.
AU - Reeders, S. T.
AU - Wilkie, A. O.M.
N1 - Funding Information:
We thank D. Callen for the cell line CY14; A.-M. Frischauf the cosmid library; Y. Nakamura for the probes CMM65, EKMDAPII, and CMM103; G. G. Germino for GGGl; and G. Scherer for Fr3-42. We also thank S. Rosevere and I. J. Goold for bringing case TA to our attention and A. Villegas for providing blood samples from patient BRV. We are grateful to S. McKeown and H. Ayyub for expert technical assistance, V. Buckle for cytogenetic analysis, and L. Rose for quality typing. D. J. Weatherall is thanked for encouragement and support. We are grateful to the Wellcome Trust and the Medical Research Council for generous support. Dr. Reeders was supported by the Howard Hughes Medical Institute and NIDDK.
PY - 1990/6
Y1 - 1990/6
N2 - Two polymorphic loci and two additional probes that map close to CMM65, which is tightly linked to the polycystic kidney disease 1 (PKD1) locus in chromosome band 16p13.3, are described. These new probes were isolated from a library that was enriched by preparative pulsed-field gel electrophoresis (PFGE) for sequences from a 320-kb NotI fragment that includes CMM65. Through the use of a panel of somatic cell hybrids and PFGE, the new polymorphic loci, PNL56S and NKISP1, were localized within 60 kb and approximately 250 kb distal to CMM65, respectively. A long-range restriction map linking these new probes and the distal markers EKMDA2, CMM103, and α-globin was constructed. These latter probes have been localized to regions approximately 900 kb, 1.2 Mb, and 1.9 Mb distal to CMM65, respectively. The entire region was found to be unusually rich in CpG dinucleotides. The new polymorphic probes and the long-range map will aid both the search for the PKD1 locus and the detailed characterization of this distal region of 16p.
AB - Two polymorphic loci and two additional probes that map close to CMM65, which is tightly linked to the polycystic kidney disease 1 (PKD1) locus in chromosome band 16p13.3, are described. These new probes were isolated from a library that was enriched by preparative pulsed-field gel electrophoresis (PFGE) for sequences from a 320-kb NotI fragment that includes CMM65. Through the use of a panel of somatic cell hybrids and PFGE, the new polymorphic loci, PNL56S and NKISP1, were localized within 60 kb and approximately 250 kb distal to CMM65, respectively. A long-range restriction map linking these new probes and the distal markers EKMDA2, CMM103, and α-globin was constructed. These latter probes have been localized to regions approximately 900 kb, 1.2 Mb, and 1.9 Mb distal to CMM65, respectively. The entire region was found to be unusually rich in CpG dinucleotides. The new polymorphic probes and the long-range map will aid both the search for the PKD1 locus and the detailed characterization of this distal region of 16p.
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U2 - 10.1016/0888-7543(90)90541-2
DO - 10.1016/0888-7543(90)90541-2
M3 - Article
C2 - 2347584
AN - SCOPUS:0025289117
SN - 0888-7543
VL - 7
SP - 195
EP - 206
JO - Genomics
JF - Genomics
IS - 2
ER -