A long-range restriction map between the α-globin complex and a marker closely linked to the polycystic kidney disease 1 (PKD1) locus

P. C. Harris, N. J. Barton, D. R. Higgs, S. T. Reeders, A. O.M. Wilkie

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

Two polymorphic loci and two additional probes that map close to CMM65, which is tightly linked to the polycystic kidney disease 1 (PKD1) locus in chromosome band 16p13.3, are described. These new probes were isolated from a library that was enriched by preparative pulsed-field gel electrophoresis (PFGE) for sequences from a 320-kb NotI fragment that includes CMM65. Through the use of a panel of somatic cell hybrids and PFGE, the new polymorphic loci, PNL56S and NKISP1, were localized within 60 kb and approximately 250 kb distal to CMM65, respectively. A long-range restriction map linking these new probes and the distal markers EKMDA2, CMM103, and α-globin was constructed. These latter probes have been localized to regions approximately 900 kb, 1.2 Mb, and 1.9 Mb distal to CMM65, respectively. The entire region was found to be unusually rich in CpG dinucleotides. The new polymorphic probes and the long-range map will aid both the search for the PKD1 locus and the detailed characterization of this distal region of 16p.

Original languageEnglish (US)
Pages (from-to)195-206
Number of pages12
JournalGenomics
Volume7
Issue number2
DOIs
StatePublished - Jun 1990

ASJC Scopus subject areas

  • Genetics

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