Abstract
The ability to deliver antigens and immunomodulators specifically to Langerhans cells (LCs) in the skin could impact vaccine development. However, cell-specific targeting of therapeutic molecules remains a challenge in biomedicine. Using phage display technologies, we have developed a protocol that identifies peptides that mediate uptake into target cell types. Employing this approach, we have isolated a 20-mer peptide that mediates specific uptake by immunopotent LCs. The peptide is functional outside the context of the phage and is able to deliver a nanoparticle to LCs in vitro. Although selected on cells in vitro, the peptide is able to direct antigens and genes to LCs in vivo. Liposomes bearing the LC targeting peptide are able to deliver a transcriptionally active gene to LCs in a mouse model. Furthermore, we demonstrate that a low-dose injection into mice of phage bearing the LC-targeting peptide yields faster and higher immune responses against phage-associated antigens than control-phage injections.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 742-752 |
| Number of pages | 11 |
| Journal | DNA and Cell Biology |
| Volume | 23 |
| Issue number | 11 |
| State | Published - Nov 2004 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Molecular Biology
- Genetics
- Cell Biology
Cite this
A library-selected, langerhans cell-targeting peptide enhances an immune response. / McGuire, Michael J.; Sykes, Kathryn F.; Samli, Kausar N.; Timares, Laura; Barry, Michael A; Stemke-Hale, Katherine; Tagliaferri, Frank; Logan, Mark; Jansa, Kimberly; Takashima, Akira; Brown, Kathlynn C.; Johnston, Stephen Albert.
In: DNA and Cell Biology, Vol. 23, No. 11, 11.2004, p. 742-752.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A library-selected, langerhans cell-targeting peptide enhances an immune response
AU - McGuire, Michael J.
AU - Sykes, Kathryn F.
AU - Samli, Kausar N.
AU - Timares, Laura
AU - Barry, Michael A
AU - Stemke-Hale, Katherine
AU - Tagliaferri, Frank
AU - Logan, Mark
AU - Jansa, Kimberly
AU - Takashima, Akira
AU - Brown, Kathlynn C.
AU - Johnston, Stephen Albert
PY - 2004/11
Y1 - 2004/11
N2 - The ability to deliver antigens and immunomodulators specifically to Langerhans cells (LCs) in the skin could impact vaccine development. However, cell-specific targeting of therapeutic molecules remains a challenge in biomedicine. Using phage display technologies, we have developed a protocol that identifies peptides that mediate uptake into target cell types. Employing this approach, we have isolated a 20-mer peptide that mediates specific uptake by immunopotent LCs. The peptide is functional outside the context of the phage and is able to deliver a nanoparticle to LCs in vitro. Although selected on cells in vitro, the peptide is able to direct antigens and genes to LCs in vivo. Liposomes bearing the LC targeting peptide are able to deliver a transcriptionally active gene to LCs in a mouse model. Furthermore, we demonstrate that a low-dose injection into mice of phage bearing the LC-targeting peptide yields faster and higher immune responses against phage-associated antigens than control-phage injections.
AB - The ability to deliver antigens and immunomodulators specifically to Langerhans cells (LCs) in the skin could impact vaccine development. However, cell-specific targeting of therapeutic molecules remains a challenge in biomedicine. Using phage display technologies, we have developed a protocol that identifies peptides that mediate uptake into target cell types. Employing this approach, we have isolated a 20-mer peptide that mediates specific uptake by immunopotent LCs. The peptide is functional outside the context of the phage and is able to deliver a nanoparticle to LCs in vitro. Although selected on cells in vitro, the peptide is able to direct antigens and genes to LCs in vivo. Liposomes bearing the LC targeting peptide are able to deliver a transcriptionally active gene to LCs in a mouse model. Furthermore, we demonstrate that a low-dose injection into mice of phage bearing the LC-targeting peptide yields faster and higher immune responses against phage-associated antigens than control-phage injections.
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UR - http://www.scopus.com/inward/citedby.url?scp=4143067588&partnerID=8YFLogxK
M3 - Article
C2 - 15585132
AN - SCOPUS:4143067588
VL - 23
SP - 742
EP - 752
JO - DNA and Cell Biology
JF - DNA and Cell Biology
SN - 1044-5498
IS - 11
ER -