A library-selected, langerhans cell-targeting peptide enhances an immune response

Michael J. McGuire, Kathryn F. Sykes, Kausar N. Samli, Laura Timares, Michael A Barry, Katherine Stemke-Hale, Frank Tagliaferri, Mark Logan, Kimberly Jansa, Akira Takashima, Kathlynn C. Brown, Stephen Albert Johnston

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The ability to deliver antigens and immunomodulators specifically to Langerhans cells (LCs) in the skin could impact vaccine development. However, cell-specific targeting of therapeutic molecules remains a challenge in biomedicine. Using phage display technologies, we have developed a protocol that identifies peptides that mediate uptake into target cell types. Employing this approach, we have isolated a 20-mer peptide that mediates specific uptake by immunopotent LCs. The peptide is functional outside the context of the phage and is able to deliver a nanoparticle to LCs in vitro. Although selected on cells in vitro, the peptide is able to direct antigens and genes to LCs in vivo. Liposomes bearing the LC targeting peptide are able to deliver a transcriptionally active gene to LCs in a mouse model. Furthermore, we demonstrate that a low-dose injection into mice of phage bearing the LC-targeting peptide yields faster and higher immune responses against phage-associated antigens than control-phage injections.

Original languageEnglish (US)
Pages (from-to)742-752
Number of pages11
JournalDNA and Cell Biology
Volume23
Issue number11
StatePublished - Nov 2004
Externally publishedYes

Fingerprint

Langerhans Cells
Libraries
Bacteriophages
Peptides
Antigens
Injections
Immunologic Factors
Liposomes
Nanoparticles
Genes
Vaccines
Technology
Skin

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

McGuire, M. J., Sykes, K. F., Samli, K. N., Timares, L., Barry, M. A., Stemke-Hale, K., ... Johnston, S. A. (2004). A library-selected, langerhans cell-targeting peptide enhances an immune response. DNA and Cell Biology, 23(11), 742-752.

A library-selected, langerhans cell-targeting peptide enhances an immune response. / McGuire, Michael J.; Sykes, Kathryn F.; Samli, Kausar N.; Timares, Laura; Barry, Michael A; Stemke-Hale, Katherine; Tagliaferri, Frank; Logan, Mark; Jansa, Kimberly; Takashima, Akira; Brown, Kathlynn C.; Johnston, Stephen Albert.

In: DNA and Cell Biology, Vol. 23, No. 11, 11.2004, p. 742-752.

Research output: Contribution to journalArticle

McGuire, MJ, Sykes, KF, Samli, KN, Timares, L, Barry, MA, Stemke-Hale, K, Tagliaferri, F, Logan, M, Jansa, K, Takashima, A, Brown, KC & Johnston, SA 2004, 'A library-selected, langerhans cell-targeting peptide enhances an immune response', DNA and Cell Biology, vol. 23, no. 11, pp. 742-752.
McGuire MJ, Sykes KF, Samli KN, Timares L, Barry MA, Stemke-Hale K et al. A library-selected, langerhans cell-targeting peptide enhances an immune response. DNA and Cell Biology. 2004 Nov;23(11):742-752.
McGuire, Michael J. ; Sykes, Kathryn F. ; Samli, Kausar N. ; Timares, Laura ; Barry, Michael A ; Stemke-Hale, Katherine ; Tagliaferri, Frank ; Logan, Mark ; Jansa, Kimberly ; Takashima, Akira ; Brown, Kathlynn C. ; Johnston, Stephen Albert. / A library-selected, langerhans cell-targeting peptide enhances an immune response. In: DNA and Cell Biology. 2004 ; Vol. 23, No. 11. pp. 742-752.
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