A lesson learned from the H3.3K27M mutation found in pediatric glioma A new approach to the study of the function of histone modifications in vivo?

Kui Ming Chan, Jing Han, Dong Fang, Haiyun Gan, Zhiguo Zhang

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Glioblastoma (GBM) is the most aggressive primary brain tumor in human. Recent studies on high-grade pediatric GBM have identified two recurrent mutations (K27M and G34R/V) in genes encoding histone H3 (H3F3A for H3.3 and HIST1H3B for H3.1).1,2 The two histone H3 mutations are mutually exclusive and give rise to tumors in different brain compartments.3 Recently, we4 and others5 have shown that the histone H3 K27M mutation specifically altered the di- and tri-methylation of endogenous histone H3 at Lys27. Genome-wide studies using ChIP-seq on H3.3K27M patient samples indicate a global reduction of H3K27me3 on chromatin. Remarkably, we also found a dramatic enrichment of H3K27me3 and EZH2 (the catalytic subunit H3K27 methyltransferase) at hundreds of gene loci in H3.3K27M patient cells. Here, we discuss potential mechanisms whereby H3K27me3 is enriched at chromatin loci in cells expressing the H3.3K27M mutation and report effects of Lys-to- Met mutations of other well-studied lysine residues of histone H3.1/H3.3 and H4 on the corresponding endogenous lysine methylation. We suggest that mutation(s) on histones may be found in a variety of human diseases, and the expression of mutant histones may help to address the function of histone lysine methylation and possibly other modifications in mammalian cells.

Original languageEnglish (US)
Pages (from-to)2546-2552
Number of pages7
JournalCell Cycle
Volume12
Issue number16
DOIs
StatePublished - Aug 15 2013

Fingerprint

Histone Code
Glioma
Histones
Pediatrics
Mutation
Methylation
Lysine
Glioblastoma
Chromatin
Methyltransferases
Brain Neoplasms
Genes
Catalytic Domain
Genome

Keywords

  • Cancer epigenetics
  • Diffuse intrinsic pontine glioma (DIPGs)
  • EZH2
  • Glioblastoma (GBM)
  • H3K27me3
  • H3K79me2
  • H4K20me3
  • Histone modification
  • PRC2 complex
  • SET-domain
  • Tumorigenesis

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Developmental Biology

Cite this

A lesson learned from the H3.3K27M mutation found in pediatric glioma A new approach to the study of the function of histone modifications in vivo? / Chan, Kui Ming; Han, Jing; Fang, Dong; Gan, Haiyun; Zhang, Zhiguo.

In: Cell Cycle, Vol. 12, No. 16, 15.08.2013, p. 2546-2552.

Research output: Contribution to journalArticle

Chan, Kui Ming ; Han, Jing ; Fang, Dong ; Gan, Haiyun ; Zhang, Zhiguo. / A lesson learned from the H3.3K27M mutation found in pediatric glioma A new approach to the study of the function of histone modifications in vivo?. In: Cell Cycle. 2013 ; Vol. 12, No. 16. pp. 2546-2552.
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