A leak pathway for luminal protons in endosomes drives oncogenic signalling in glioblastoma

Kalyan C. Kondapalli, Jose P. Llongueras, Vivian Capilla-González, Hari Prasad, Anniesha Hack, Christopher Smith, Hugo Guerrero Cazares, Alfredo Quinones-Hinojosa, Rajini Rao

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Epidermal growth factor receptor (EGFR) signalling is a potent driver of glioblastoma, a malignant and lethal form of brain cancer. Disappointingly, inhibitors targeting receptor tyrosine kinase activity are not clinically effective and EGFR persists on the plasma membrane to maintain tumour growth and invasiveness. Here we show that endolysosomal pH is critical for receptor sorting and turnover. By functioning as a leak pathway for protons, the Na+/H+ exchanger NHE9 limits luminal acidification to circumvent EGFR turnover and prolong downstream signalling pathways that drive tumour growth and migration. In glioblastoma, NHE9 expression is associated with stem/progenitor characteristics, radiochemoresistance, poor prognosis and invasive growth in vitro and in vivo. Silencing or inhibition of NHE9 in brain tumour-initiating cells attenuates tumoursphere formation and improves efficacy of EGFR inhibitor. Thus, NHE9 mediates inside-out control of oncogenic signalling and is a highly druggable target for pan-specific receptor clearance in cancer therapy.

Original languageEnglish (US)
Article number6289
JournalNature Communications
Volume6
DOIs
StatePublished - Feb 9 2015
Externally publishedYes

Fingerprint

Endosomes
Glioblastoma
Epidermal Growth Factor Receptor
Protons
Tumors
protons
Brain Neoplasms
Brain
Growth
tumors
Neoplasms
Sodium-Hydrogen Antiporter
Neoplastic Stem Cells
Acidification
Receptor Protein-Tyrosine Kinases
inhibitors
Cell membranes
brain
Sorting
cancer

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Kondapalli, K. C., Llongueras, J. P., Capilla-González, V., Prasad, H., Hack, A., Smith, C., ... Rao, R. (2015). A leak pathway for luminal protons in endosomes drives oncogenic signalling in glioblastoma. Nature Communications, 6, [6289]. https://doi.org/10.1038/ncomms7289

A leak pathway for luminal protons in endosomes drives oncogenic signalling in glioblastoma. / Kondapalli, Kalyan C.; Llongueras, Jose P.; Capilla-González, Vivian; Prasad, Hari; Hack, Anniesha; Smith, Christopher; Guerrero Cazares, Hugo; Quinones-Hinojosa, Alfredo; Rao, Rajini.

In: Nature Communications, Vol. 6, 6289, 09.02.2015.

Research output: Contribution to journalArticle

Kondapalli KC, Llongueras JP, Capilla-González V, Prasad H, Hack A, Smith C et al. A leak pathway for luminal protons in endosomes drives oncogenic signalling in glioblastoma. Nature Communications. 2015 Feb 9;6. 6289. https://doi.org/10.1038/ncomms7289
Kondapalli, Kalyan C. ; Llongueras, Jose P. ; Capilla-González, Vivian ; Prasad, Hari ; Hack, Anniesha ; Smith, Christopher ; Guerrero Cazares, Hugo ; Quinones-Hinojosa, Alfredo ; Rao, Rajini. / A leak pathway for luminal protons in endosomes drives oncogenic signalling in glioblastoma. In: Nature Communications. 2015 ; Vol. 6.
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