TY - JOUR
T1 - A human alphoid DNA clone from the EcoRI dimeric family
T2 - Genomic and internal organization and chromosomal assignment
AU - Baldini, A.
AU - Smith, D. I.
AU - Rocchi, M.
AU - Miller, O. J.
AU - Miller, D. A.
N1 - Funding Information:
This work was supported by grants from the March of Dimes Birth Defects Foundation (I-950 to D.A.M.), the National Institutes of Health (GM 39788 and GM 34959 to O.J.M.), and the Center for Molecular Biology (to M.R., D.I.S., and O.J.M.).
PY - 1989/11
Y1 - 1989/11
N2 - We isolated an alpha satellite DNA clone (pC1.8), 17 kb long, which is composed exclusively of tandemly repeated 340-bp EcoRI fragments. Hybridization studies using 37 random EcoRI dimers subcloned from pC1.8 showed that they are heterogeneous. The sequence of 5 dimers, 3 of them adjacent, confirmed this observation and showed that the heterogeneity is more accentuated among the second monomers. The chromosomal assignment under high stringency conditions showed that this alphoid subset is located on chromosomes 1, 5, and 19. No conditions that eliminate the hybridization on any one of those chromosomes were found. This suggests that, in contrast to many other chromosome-specific alpha satellite subsets, the single chromosome subsets of this family are virtually indistinguishable by hybridization techniques.
AB - We isolated an alpha satellite DNA clone (pC1.8), 17 kb long, which is composed exclusively of tandemly repeated 340-bp EcoRI fragments. Hybridization studies using 37 random EcoRI dimers subcloned from pC1.8 showed that they are heterogeneous. The sequence of 5 dimers, 3 of them adjacent, confirmed this observation and showed that the heterogeneity is more accentuated among the second monomers. The chromosomal assignment under high stringency conditions showed that this alphoid subset is located on chromosomes 1, 5, and 19. No conditions that eliminate the hybridization on any one of those chromosomes were found. This suggests that, in contrast to many other chromosome-specific alpha satellite subsets, the single chromosome subsets of this family are virtually indistinguishable by hybridization techniques.
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U2 - 10.1016/0888-7543(89)90124-9
DO - 10.1016/0888-7543(89)90124-9
M3 - Article
C2 - 2591965
AN - SCOPUS:0024758528
SN - 0888-7543
VL - 5
SP - 822
EP - 828
JO - Genomics
JF - Genomics
IS - 4
ER -