A highly immunogenic region of a human polymorphic epithelial mucin expressed by carcinomas is made up of tandem repeats

Sandra J Gendler, J. Taylor-Papadimitriou, T. Duhig, J. Rothbard, J. Burchel

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Abstract

The nucleotide sequences of partial cDNA clones coding for the core protein of a human polymorphic epithelial mucin were determined, and a large domain was found to consist of a 60-base pair tandem repeat sequence. The cDNA clones were originally selected (Gendler, S.J., Burchell, J.M., Duhig, T., Lamport, D., White, R., Parker, M., and Tailor-Papadimitriou, J. (1987) Proc. Natl. Acad. Sci. U.S.A. 84, 6060-6064) using three monoclonal antibodies which show differential reactivity with the mucin produced by normal and malignant breast. Two of the epitopes are exposed in the normally processed and cancer-associated mucin, while one epitope is unmasked only in the cancer-associated mucin (Burchell, J.M., Durbin, H., and Taylor-Papadimitriou, J. (1983) J. Immunol. 131, 508-513; Burchell, J., Gendler, S., Taylor-Papadimitriou, J., Girling, A., Lewis, A., Millis, R., and Lamport, D. (1987) Cancer Res. 47, 5476-5482). We show here that all three antibodies react with a synthetic peptide with an amino acid sequence corresponding to that predicted by the tandem repeat. Identification of the epitopes preferentially expressed on the cancer-associated mucin should allow a directed approach to the development of tumor-specific antibodies using synthetic peptides as immunogens.

Original languageEnglish (US)
Pages (from-to)12820-12823
Number of pages4
JournalJournal of Biological Chemistry
Volume263
Issue number26
StatePublished - 1988
Externally publishedYes

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Mucin-1
Tandem Repeat Sequences
Mucins
Carcinoma
Epitopes
Neoplasms
Complementary DNA
Clone Cells
Neoplasm Antibodies
Peptides
Antibodies
Base Pairing
Tumors
Amino Acid Sequence
Breast
Nucleotides
Monoclonal Antibodies
Amino Acids
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

A highly immunogenic region of a human polymorphic epithelial mucin expressed by carcinomas is made up of tandem repeats. / Gendler, Sandra J; Taylor-Papadimitriou, J.; Duhig, T.; Rothbard, J.; Burchel, J.

In: Journal of Biological Chemistry, Vol. 263, No. 26, 1988, p. 12820-12823.

Research output: Contribution to journalArticle

Gendler, SJ, Taylor-Papadimitriou, J, Duhig, T, Rothbard, J & Burchel, J 1988, 'A highly immunogenic region of a human polymorphic epithelial mucin expressed by carcinomas is made up of tandem repeats', Journal of Biological Chemistry, vol. 263, no. 26, pp. 12820-12823.
Gendler, Sandra J ; Taylor-Papadimitriou, J. ; Duhig, T. ; Rothbard, J. ; Burchel, J. / A highly immunogenic region of a human polymorphic epithelial mucin expressed by carcinomas is made up of tandem repeats. In: Journal of Biological Chemistry. 1988 ; Vol. 263, No. 26. pp. 12820-12823.
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