A highly conserved intronic sequence is involved in transcriptional regulation of the alpha 1(I) collagen gene.

D. J. Liska, James L Slack, P. Bornstein

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The first intron of the human alpha 1(I) collagen gene contains a positive, orientation-dependent cis-acting sequence located between bases +292 and +670. Transient transfection experiments indicate that this sequence is functional in both primary chicken tendon fibroblasts and in a human fibroblast-like cell line derived from SV40-transformed marrow stromal cells. DNase I footprint, methylation interference, and mobility shift analyses provide evidence for a sequence-specific binding activity and show that the region of binding corresponds to a 29-base-pair sequence that is also present in the rat alpha 1(I) collagen intron. This conserved sequence contains an AP1 consensus motif. Sequence-specific binding activity is present in nuclear extracts from HeLa and fibroblast cell lines but not in extracts from two lymphoid cell lines. Mutation of the AP1 consensus sequence indicates that this motif is required for function of the cis-acting element. These data indicate that transcriptional modulation of the alpha 1(I) collagen gene involves an interaction between an intronic AP1-containing sequence and its cognate transcription factors.

Original languageEnglish (US)
Pages (from-to)487-498
Number of pages12
JournalCell regulation
Volume1
Issue number6
StatePublished - May 1990
Externally publishedYes

Fingerprint

Conserved Sequence
Collagen
Fibroblasts
Cell Line
Introns
Genes
Deoxyribonuclease I
Consensus Sequence
Stromal Cells
HeLa Cells
Base Pairing
Tendons
Methylation
Transfection
Chickens
Transcription Factors
Bone Marrow
Lymphocytes
Mutation

ASJC Scopus subject areas

  • Medicine(all)

Cite this

A highly conserved intronic sequence is involved in transcriptional regulation of the alpha 1(I) collagen gene. / Liska, D. J.; Slack, James L; Bornstein, P.

In: Cell regulation, Vol. 1, No. 6, 05.1990, p. 487-498.

Research output: Contribution to journalArticle

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