A high-throughput 3-parameter flow cytometry-based cell death assay

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Apoptosis plays a role in many disease states, and the evaluation of novel therapeutics that alters the apoptotic cascade is an area of intense investigation. However, many generally available methods to evaluate cell death are either time consuming, imprecise, or both. We report a system that permits simultaneous evaluation of three apoptotic markers (cell membrane integrity, mitochondrial membrane potential, and cell cycle progression) with minimal technical manipulation. This system is particularly well-suited for toxicologic evaluation of novel compounds and profiling of new apoptosis-inducing agents.

Original languageEnglish (US)
Pages (from-to)170-173
Number of pages4
JournalCytometry Part A
Volume71
Issue number3
DOIs
StatePublished - Mar 1 2007

Keywords

  • Apoptosis
  • Cell death
  • Drug discovery
  • High-throughout screen
  • Mitochondria
  • Necrosis

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

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