A genome-wide association study of venous thromboembolism identifies risk variants in chromosomes 1q24.2 and 9q

J. A. Heit, S. M. Armasu, Yan Asmann, Julie M Cunningham, M. E. Matsumoto, T. M. Petterson, Mariza De Andrade

Research output: Contribution to journalArticle

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Abstract

Objectives: To identify venous thromboembolism (VTE) disease-susceptibility genes. Patients and methods: We performed in silico genome wide association scan (GWAS) analyses using genotype data imputed to approximately 2.5million single-nucleotide polymorphisms (SNPs) from adults with objectively-diagnosed VTE (n=1503), and controls frequency matched on age and gender (n=1459; discovery population). Single-nucleotide polymorphisms exceeding genome-wide significance were replicated in a separate population (VTE cases, n = 1407; controls, n = 1418). Genes associated with VTE were re-sequenced. Results: Seven SNPs exceeded genome-wide significance (P<5×10-8): four on chromosome 1q24.2 (F5 rs6025 [factor V Leiden], BLZF1 rs7538157, NME7 rs16861990 and SLC19A2 rs2038024) and three on chromosome 9q34.2 (ABO rs2519093 [ABO intron 1], rs495828, rs8176719 [ABO blood type O allele]). The replication study confirmed a significant association of F5, NME7 and ABO with VTE. However, F5 was the main signal on 1q24.2 as only ABO SNPs remained significantly associated with VTE after adjusting for F5 rs6025. This 1q24.2 region was shown to be inherited as a haplotype block. ABO re-sequencing identified 15 novel single nucleotide variations (SNV) in ABO intron 6 and the ABO 3′ UTR that were strongly associated with VTE (P<10-4) and belonged to three distinct linkage disequilibrium (LD) blocks; none were in LD with ABO rs8176719 or rs2519093. Our sample size provided 80% power to detect odds ratios (ORs)=2.0 and 1.51 for minor allele frequencies=0.05 and 0.5, respectively (α = 1 ×10-8; 1% VTE prevalence). Conclusions: Apart from F5 rs6025, ABO rs8176719, rs2519093 and F2 rs1799963, additional common and high VTE-risk SNPs among whites are unlikely.

Original languageEnglish (US)
Pages (from-to)1521-1531
Number of pages11
JournalJournal of Thrombosis and Haemostasis
Volume10
Issue number8
DOIs
StatePublished - Aug 2012

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Genome-Wide Association Study
Venous Thromboembolism
Chromosomes
Single Nucleotide Polymorphism
Linkage Disequilibrium
Introns
Genome
Disease Susceptibility
3' Untranslated Regions
Gene Frequency
Computer Simulation
Sample Size
Haplotypes
Population
Genes
Nucleotides
Alleles
Odds Ratio
Genotype

Keywords

  • Deep vein thrombosis
  • Epidemiology
  • Genetics
  • Genome-wide scan
  • Pulmonary embolism
  • Venous thromboembolism

ASJC Scopus subject areas

  • Hematology

Cite this

A genome-wide association study of venous thromboembolism identifies risk variants in chromosomes 1q24.2 and 9q. / Heit, J. A.; Armasu, S. M.; Asmann, Yan; Cunningham, Julie M; Matsumoto, M. E.; Petterson, T. M.; De Andrade, Mariza.

In: Journal of Thrombosis and Haemostasis, Vol. 10, No. 8, 08.2012, p. 1521-1531.

Research output: Contribution to journalArticle

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abstract = "Objectives: To identify venous thromboembolism (VTE) disease-susceptibility genes. Patients and methods: We performed in silico genome wide association scan (GWAS) analyses using genotype data imputed to approximately 2.5million single-nucleotide polymorphisms (SNPs) from adults with objectively-diagnosed VTE (n=1503), and controls frequency matched on age and gender (n=1459; discovery population). Single-nucleotide polymorphisms exceeding genome-wide significance were replicated in a separate population (VTE cases, n = 1407; controls, n = 1418). Genes associated with VTE were re-sequenced. Results: Seven SNPs exceeded genome-wide significance (P<5×10-8): four on chromosome 1q24.2 (F5 rs6025 [factor V Leiden], BLZF1 rs7538157, NME7 rs16861990 and SLC19A2 rs2038024) and three on chromosome 9q34.2 (ABO rs2519093 [ABO intron 1], rs495828, rs8176719 [ABO blood type O allele]). The replication study confirmed a significant association of F5, NME7 and ABO with VTE. However, F5 was the main signal on 1q24.2 as only ABO SNPs remained significantly associated with VTE after adjusting for F5 rs6025. This 1q24.2 region was shown to be inherited as a haplotype block. ABO re-sequencing identified 15 novel single nucleotide variations (SNV) in ABO intron 6 and the ABO 3′ UTR that were strongly associated with VTE (P<10-4) and belonged to three distinct linkage disequilibrium (LD) blocks; none were in LD with ABO rs8176719 or rs2519093. Our sample size provided 80{\%} power to detect odds ratios (ORs)=2.0 and 1.51 for minor allele frequencies=0.05 and 0.5, respectively (α = 1 ×10-8; 1{\%} VTE prevalence). Conclusions: Apart from F5 rs6025, ABO rs8176719, rs2519093 and F2 rs1799963, additional common and high VTE-risk SNPs among whites are unlikely.",
keywords = "Deep vein thrombosis, Epidemiology, Genetics, Genome-wide scan, Pulmonary embolism, Venous thromboembolism",
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T1 - A genome-wide association study of venous thromboembolism identifies risk variants in chromosomes 1q24.2 and 9q

AU - Heit, J. A.

AU - Armasu, S. M.

AU - Asmann, Yan

AU - Cunningham, Julie M

AU - Matsumoto, M. E.

AU - Petterson, T. M.

AU - De Andrade, Mariza

PY - 2012/8

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N2 - Objectives: To identify venous thromboembolism (VTE) disease-susceptibility genes. Patients and methods: We performed in silico genome wide association scan (GWAS) analyses using genotype data imputed to approximately 2.5million single-nucleotide polymorphisms (SNPs) from adults with objectively-diagnosed VTE (n=1503), and controls frequency matched on age and gender (n=1459; discovery population). Single-nucleotide polymorphisms exceeding genome-wide significance were replicated in a separate population (VTE cases, n = 1407; controls, n = 1418). Genes associated with VTE were re-sequenced. Results: Seven SNPs exceeded genome-wide significance (P<5×10-8): four on chromosome 1q24.2 (F5 rs6025 [factor V Leiden], BLZF1 rs7538157, NME7 rs16861990 and SLC19A2 rs2038024) and three on chromosome 9q34.2 (ABO rs2519093 [ABO intron 1], rs495828, rs8176719 [ABO blood type O allele]). The replication study confirmed a significant association of F5, NME7 and ABO with VTE. However, F5 was the main signal on 1q24.2 as only ABO SNPs remained significantly associated with VTE after adjusting for F5 rs6025. This 1q24.2 region was shown to be inherited as a haplotype block. ABO re-sequencing identified 15 novel single nucleotide variations (SNV) in ABO intron 6 and the ABO 3′ UTR that were strongly associated with VTE (P<10-4) and belonged to three distinct linkage disequilibrium (LD) blocks; none were in LD with ABO rs8176719 or rs2519093. Our sample size provided 80% power to detect odds ratios (ORs)=2.0 and 1.51 for minor allele frequencies=0.05 and 0.5, respectively (α = 1 ×10-8; 1% VTE prevalence). Conclusions: Apart from F5 rs6025, ABO rs8176719, rs2519093 and F2 rs1799963, additional common and high VTE-risk SNPs among whites are unlikely.

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KW - Epidemiology

KW - Genetics

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KW - Pulmonary embolism

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