A genome-specific repetitive DNA sequence from Oryza eichingeri: Characterization, localization, and introgression to O. sativa

H. H. Yan, G. Q. Liu, Z. K. Cheng, X. B. Li, G. Z. Liu, S. K. Min, L. H. Zhu

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

In the course of transferring the brown planthopper resistance from a diploid, CC-genome wild rice species, Oryza eichingeri (IRGC acc. 105159 and 105163), to the cultivated rice variety 02428, we have isolated many alien addition and introgression lines. The O. eichingeri chromatin in some of these lines has previously been identified using genomic in situ hybridization and molecular-marker analysis. Here we cloned a tandemly repetitive DNA sequence from O. eichingeri IRGC acc105163, and detected it in 25 introgression lines. This repetitive DNA sequence showed high specificity to the rice CC genome, but was absent from all the four tetraploid species with BBCC or CCDD genomes. The monomer in this repetitive DNA sequence is 325-366-bp long, with a copy number of about 5,000 per 1 C of the O. eichingeri genome, showing 88% homology to a repetitive DNA sequence isolated from Oryza officinalis (2n=2x=24, CC). Fluorescent in situ hybridization revealed 11 signals distributed over eight O. eichingeri chromosomes, mostly in terminal or subterminal regions.

Original languageEnglish (US)
Pages (from-to)177-183
Number of pages7
JournalTheoretical and Applied Genetics
Volume104
Issue number2-3
DOIs
StatePublished - Dec 1 2002

Keywords

  • Fluorescent in situ hybridization
  • Introgression lines
  • Oryza eichingeri
  • Repetitive DNA sequence

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

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