TY - JOUR
T1 - A genome-specific repetitive DNA sequence from Oryza eichingeri
T2 - Characterization, localization, and introgression to O. sativa
AU - Yan, H. H.
AU - Liu, G. Q.
AU - Cheng, Z. K.
AU - Li, X. B.
AU - Liu, G. Z.
AU - Min, S. K.
AU - Zhu, L. H.
PY - 2002
Y1 - 2002
N2 - In the course of transferring the brown planthopper resistance from a diploid, CC-genome wild rice species, Oryza eichingeri (IRGC acc. 105159 and 105163), to the cultivated rice variety 02428, we have isolated many alien addition and introgression lines. The O. eichingeri chromatin in some of these lines has previously been identified using genomic in situ hybridization and molecular-marker analysis. Here we cloned a tandemly repetitive DNA sequence from O. eichingeri IRGC acc105163, and detected it in 25 introgression lines. This repetitive DNA sequence showed high specificity to the rice CC genome, but was absent from all the four tetraploid species with BBCC or CCDD genomes. The monomer in this repetitive DNA sequence is 325-366-bp long, with a copy number of about 5,000 per 1 C of the O. eichingeri genome, showing 88% homology to a repetitive DNA sequence isolated from Oryza officinalis (2n=2x=24, CC). Fluorescent in situ hybridization revealed 11 signals distributed over eight O. eichingeri chromosomes, mostly in terminal or subterminal regions.
AB - In the course of transferring the brown planthopper resistance from a diploid, CC-genome wild rice species, Oryza eichingeri (IRGC acc. 105159 and 105163), to the cultivated rice variety 02428, we have isolated many alien addition and introgression lines. The O. eichingeri chromatin in some of these lines has previously been identified using genomic in situ hybridization and molecular-marker analysis. Here we cloned a tandemly repetitive DNA sequence from O. eichingeri IRGC acc105163, and detected it in 25 introgression lines. This repetitive DNA sequence showed high specificity to the rice CC genome, but was absent from all the four tetraploid species with BBCC or CCDD genomes. The monomer in this repetitive DNA sequence is 325-366-bp long, with a copy number of about 5,000 per 1 C of the O. eichingeri genome, showing 88% homology to a repetitive DNA sequence isolated from Oryza officinalis (2n=2x=24, CC). Fluorescent in situ hybridization revealed 11 signals distributed over eight O. eichingeri chromosomes, mostly in terminal or subterminal regions.
KW - Fluorescent in situ hybridization
KW - Introgression lines
KW - Oryza eichingeri
KW - Repetitive DNA sequence
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U2 - 10.1007/s001220100766
DO - 10.1007/s001220100766
M3 - Article
AN - SCOPUS:0036939392
SN - 0040-5752
VL - 104
SP - 177
EP - 183
JO - Theoretical and Applied Genetics
JF - Theoretical and Applied Genetics
IS - 2-3
ER -