A functional single-nucleotide polymorphism in the promoter of the gene encoding interleukin 6 is associated with susceptibility to tuberculosis

Guoliang Zhang, Boping Zhou, Wenfei Wang, Mingxia Zhang, Yahua Zhao, Zheng Wang, Lin Yang, Jingnan Zhai, Carl G. Feng, Junwen Wang, Xinchun Chen

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32 Scopus citations

Abstract

Background Genetic variation influences susceptibility or resistance to tuberculosis. Interleukin 6 (IL-6) contributes to protection against tuberculosis in mice. However, its role in regulating susceptibility or resistance to tuberculosis in humans is unclear.MethodsGenotyping of polymorphisms in IL-6 and IL-6R (CD126) genes was performed in 2 independent cohorts, an experimental population (495 cases and 358 controls) and a validation population (1383 cases and 1149 controls). The associations of the variants with tuberculosis were tested using 2 case-control association studies. In addition, the regulatory effects of single-nucleotide polymorphism rs1800796 (-572C > G) on IL-6 production in plasma and CD14 + monocyte cultures stimulated with a Mycobacterium tuberculosis (M. tuberculosis) product were assessed.ResultsThe rs1800796 polymorphism is associated with increased resistance to tuberculosis (odds ratio [OR], 0.771; 95 confidential interval,. 684-.870). The rs1800796GG genotype is strongly associated with reduced risk to tuberculosis (OR, 0.621; 95 CI,. 460-.838). Interestingly, CD14 + monocytes isolated from individuals with rs1800796GG genotype produced significantly less IL-6 in response to M. tuberculosis 19-kDa lipoprotein than those with CC or CG genotype. Conclusions We identified a genetic polymorphism in the IL-6 promoter that regulates cytokine production and host resistance to pulmonary tuberculosis in Chinese populations.

Original languageEnglish (US)
Pages (from-to)1697-1704
Number of pages8
JournalJournal of Infectious Diseases
Volume205
Issue number11
DOIs
StatePublished - Jun 1 2012

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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