A fragment of the hypophosphatemic factor, MEPE, requires inducible cyclooxygenase-2 to exert potent anabolic effects on normal human marrow osteoblast precursors

D. E. Nagel, Sundeep Khosla, A. Sanyal, D. M. Rosen, Y. Kumagai, B. Lawrence Riggs

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

MEPE, 56.6 kDa protein isolated from tumors associated with hypophosphatemic osteomalacia, increases renal phosphate excretion and is expressed in normal human bone cells. AC-100, a central 23-amino acid fragment of MEPE, contains motifs that are important in regulating cellular activities in the bone microenvironment. Thus, we assessed in vitro effects of AC-100 on multipotential normal human marrow stromal (hMS) cells that have the capacity to differentiate into mature osteoblasts. Proliferation was quantified by [H 3]thymidine uptake and cell counting and differentiation by the levels of mRNA for the α2-chain of type I procollagen (COL1A2), alkaline phosphatase (AP), and osteocalcin (OC) measured using real time reverse transcriptase PCR (RT-PCR) and by the formation of mineralized nodules. AC-100 increased proliferation by 257 ± 89% (P < 0.005), increased gene expression of COL1A2 by 339 ± 85% (P < 0.005), AP by 1,437 ± 40% (P < 0.001), and OC by 1,962 ± 337% (P < 0.001). In addition, it increased mineralized nodule formation by 81 ± 14% (P < 0.001) in a dose- and time-dependent fashion. In equimolar dosages, the parent compound, MEPE, had the full activity of the AC-100 fragment. AC-100 elicited a comparable response to both IGF-1 and BMP-2 with respect to proliferation and differentiation of hMS cells. Using gene expression microarray analysis, we demonstrated that AC-100 increased (by ∼3-fold) the mRNA for cyclooxgenase-2 (COX-2), an inducible enzyme required for prostaglandin synthesis. Moreover, NS-398, a specific inhibitor of COX-2 action completely blocked AC-100-induced increases in proliferation and differentiation. Thus, AC-100 has potent anabolic activity on osteoblast precursor cells in vitro and these effects require the induction of COX-2.

Original languageEnglish (US)
Pages (from-to)1107-1114
Number of pages8
JournalJournal of Cellular Biochemistry
Volume93
Issue number6
DOIs
StatePublished - 2004

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Keywords

  • Anabolic agent
  • BMP-2
  • Bone formation
  • COX-2

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

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