A dynamic approach reveals non-muscle myosin influences the overall smooth muscle cross-bridge cycling rate

Bilge Guvenc; Cansel Ustunel; Necla Ozturk; Frank V. Brozovich

doi:10.1016/j.febslet.2010.04.070

A dynamic approach reveals non-muscle myosin influences the overall smooth muscle cross-bridge cycling rate

Bilge Guvenc, Cansel Ustunel, Necla Ozturk, Frank V. Brozovich

Cardiovascular Medicine

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Recent evidence suggests that non-muscle myosin IIB (NMIIB) contributes to smooth muscle contraction. This study was designed to determine the effects of NMIIB on the cross-bridge cycling rate. The cross-bridge cycling rate was investigated using sinusoidal analysis. Frequency analysis revealed two asymptotes in the Bode plot of the data; and the intersection of the asymptotes (corner frequency) was higher for the B^+/- strain (8.73±1.10Hz vs 16.56±1.26Hz, P<0.05), consistent with a higher overall cross-bridge cycling rate in heterozygous NMIIB KO (B^+/-) vs WT mice. These results demonstrate that because of their long duty cycle, NMIIB cross-bridges act as an internal load on smooth muscle myosin to decrease the overall cross-bridge cycling rate and muscle V_max during force maintenance.

Original language	English (US)
Pages (from-to)	2862-2866
Number of pages	5
Journal	FEBS Letters
Volume	584
Issue number	13
DOIs	https://doi.org/10.1016/j.febslet.2010.04.070
State	Published - Jul 2010

Keywords

Contractile phenotype
Force maintenance
Latch
Phasic
Tonic

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2010.04.070

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title = "A dynamic approach reveals non-muscle myosin influences the overall smooth muscle cross-bridge cycling rate",

abstract = "Recent evidence suggests that non-muscle myosin IIB (NMIIB) contributes to smooth muscle contraction. This study was designed to determine the effects of NMIIB on the cross-bridge cycling rate. The cross-bridge cycling rate was investigated using sinusoidal analysis. Frequency analysis revealed two asymptotes in the Bode plot of the data; and the intersection of the asymptotes (corner frequency) was higher for the B+/- strain (8.73±1.10Hz vs 16.56±1.26Hz, P<0.05), consistent with a higher overall cross-bridge cycling rate in heterozygous NMIIB KO (B+/-) vs WT mice. These results demonstrate that because of their long duty cycle, NMIIB cross-bridges act as an internal load on smooth muscle myosin to decrease the overall cross-bridge cycling rate and muscle Vmax during force maintenance.",

keywords = "Contractile phenotype, Force maintenance, Latch, Phasic, Tonic",

author = "Bilge Guvenc and Cansel Ustunel and Necla Ozturk and Brozovich, {Frank V.}",

note = "Funding Information: We wish to thank Dr. Ozgur Ogut for his comments on the manuscript and valuable advise. This study was supported by a research grant from the Mayo Clinic. ",

year = "2010",

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AU - Guvenc, Bilge

AU - Ustunel, Cansel

AU - Ozturk, Necla

AU - Brozovich, Frank V.

N1 - Funding Information: We wish to thank Dr. Ozgur Ogut for his comments on the manuscript and valuable advise. This study was supported by a research grant from the Mayo Clinic.

PY - 2010/7

Y1 - 2010/7

N2 - Recent evidence suggests that non-muscle myosin IIB (NMIIB) contributes to smooth muscle contraction. This study was designed to determine the effects of NMIIB on the cross-bridge cycling rate. The cross-bridge cycling rate was investigated using sinusoidal analysis. Frequency analysis revealed two asymptotes in the Bode plot of the data; and the intersection of the asymptotes (corner frequency) was higher for the B+/- strain (8.73±1.10Hz vs 16.56±1.26Hz, P<0.05), consistent with a higher overall cross-bridge cycling rate in heterozygous NMIIB KO (B+/-) vs WT mice. These results demonstrate that because of their long duty cycle, NMIIB cross-bridges act as an internal load on smooth muscle myosin to decrease the overall cross-bridge cycling rate and muscle Vmax during force maintenance.

AB - Recent evidence suggests that non-muscle myosin IIB (NMIIB) contributes to smooth muscle contraction. This study was designed to determine the effects of NMIIB on the cross-bridge cycling rate. The cross-bridge cycling rate was investigated using sinusoidal analysis. Frequency analysis revealed two asymptotes in the Bode plot of the data; and the intersection of the asymptotes (corner frequency) was higher for the B+/- strain (8.73±1.10Hz vs 16.56±1.26Hz, P<0.05), consistent with a higher overall cross-bridge cycling rate in heterozygous NMIIB KO (B+/-) vs WT mice. These results demonstrate that because of their long duty cycle, NMIIB cross-bridges act as an internal load on smooth muscle myosin to decrease the overall cross-bridge cycling rate and muscle Vmax during force maintenance.

KW - Contractile phenotype

KW - Force maintenance

KW - Latch

KW - Phasic

KW - Tonic

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A dynamic approach reveals non-muscle myosin influences the overall smooth muscle cross-bridge cycling rate

Abstract

Keywords

ASJC Scopus subject areas

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