A dynamic approach reveals non-muscle myosin influences the overall smooth muscle cross-bridge cycling rate

Bilge Guvenc, Cansel Ustunel, Necla Ozturk, Frank V. Brozovich

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Recent evidence suggests that non-muscle myosin IIB (NMIIB) contributes to smooth muscle contraction. This study was designed to determine the effects of NMIIB on the cross-bridge cycling rate. The cross-bridge cycling rate was investigated using sinusoidal analysis. Frequency analysis revealed two asymptotes in the Bode plot of the data; and the intersection of the asymptotes (corner frequency) was higher for the B+/- strain (8.73±1.10Hz vs 16.56±1.26Hz, P<0.05), consistent with a higher overall cross-bridge cycling rate in heterozygous NMIIB KO (B+/-) vs WT mice. These results demonstrate that because of their long duty cycle, NMIIB cross-bridges act as an internal load on smooth muscle myosin to decrease the overall cross-bridge cycling rate and muscle Vmax during force maintenance.

Original languageEnglish (US)
Pages (from-to)2862-2866
Number of pages5
JournalFEBS Letters
Volume584
Issue number13
DOIs
StatePublished - Jul 1 2010

Keywords

  • Contractile phenotype
  • Force maintenance
  • Latch
  • Phasic
  • Tonic

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Fingerprint Dive into the research topics of 'A dynamic approach reveals non-muscle myosin influences the overall smooth muscle cross-bridge cycling rate'. Together they form a unique fingerprint.

  • Cite this