A Dyn2-CIN85 complex mediates degradative traffic of the EGFR by regulation of late endosomal budding

Barbara Schroeder, Shaun G. Weller, Jing Chen, Daniel Billadeau, Mark A. McNiven

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

The epidermal growth factor receptor (EGFR) is over-expressed in a variety of human cancers. Downstream signalling of this receptor is tightly regulated both spatially and temporally by controlling its internalization and subsequent degradation. Internalization of the EGFR requires dynamin 2 (Dyn2), a large GTPase that deforms lipid bilayers, leading to vesicle scission. The adaptor protein CIN85 (cbl-interacting protein of 85 kDa), which has been proposed to indirectly link the EGFR to the endocytic machinery at the plasma membrane, is also thought to be involved in receptor internalization. Here, we report a novel and direct interaction between Dyn2 and CIN85 that is induced by EGFR stimulation and, most surprisingly, occurs late in the endocytic process. Importantly, disruption of the CIN85-Dyn2 interaction results in accumulation of internalized EGFR in late endosomes that become aberrantly elongated into distended tubules. Consistent with the accumulation of this receptor is a sustention of downstream signalling cascades. These findings provide novel insights into a previously unknown protein complex that can regulate EGFR traffic at very late stages of the endocytic pathway.

Original languageEnglish (US)
Pages (from-to)3039-3053
Number of pages15
JournalEMBO Journal
Volume29
Issue number18
DOIs
StatePublished - Sep 2010

Keywords

  • CIN85-Dyn2 complex
  • EGFR trafficking
  • late endosomal dynamics

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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