A doxorubicin-induced cardiomyopathy model in adult zebrafish

Xiao Ma, Yonghe Ding, Yong Wang, Xiaolei Xu

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

The genetically accessible adult zebrafish (Danio rerio) has been increasingly used as a vertebrate model for understanding human diseases such as cardiomyopathy. Because of its convenience and amenability to high throughput genetic manipulations, the generation of acquired cardiomyopathy models, such as the doxorubicin-induced cardiomyopathy (DIC) model in adult zebrafish, is opening the doors to new research avenues, including discovering cardiomyopathy modifiers via forward genetic screening. Different from the embryonic zebrafish DIC model, both initial acute and later chronic phases of cardiomyopathy can be determined in the adult zebrafish DIC model, enabling the study of stage-dependent signaling mechanisms and therapeutic strategies. However, variable results can be obtained with the current model, even in the hands of experienced investigators. To facilitate future implementation of the DIC model, we present a detailed protocol on how to generate this DIC model in adult zebrafish and describe two alternative ways of intraperitoneal (IP) injection. We further discuss options on how to reduce variations to obtain reliable results and provide suggestions on how to appropriately interpret the results.

Original languageEnglish (US)
Article numbere57567
JournalJournal of Visualized Experiments
Volume2018
Issue number136
DOIs
StatePublished - Jun 7 2018

Keywords

  • Acute phase
  • Cardiomyopathy
  • Chronic phase
  • Disease model
  • Doxorubicin
  • Intraperitoneal injection
  • Issue 136
  • Medicine
  • Zebrafish

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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