A DNA probe suitable for the detection of chromosome 21 copy number in human interphase nuclei by fluorescence in situ hybridization

Q. Shi, X. Shan, J. Zhang, X. Zhang, Y. Cheng, X. Dang, Haojie Huang, L. Yu, S. Zhao, Q. Zheng, A. Id

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Objective To prepare DNA probe which can be used to accurately detect chromosome 21 copy number in human interphase nuclei by fluorescence in situ hybridization (FISH). Methods A probe was produced by universal primer PCR from YAC clone 881D2 and characterized by FISH to metaphases and interphase nuclei of peripheral lymphocytes from 8 normal individuals and 5 patients suffering from trisomy 21. Results A DNA probe was produced and its characteristics were: (1) Most of the probes ranged in size from 350bp to 750bp; (2) The hybridization signals of the probes were located near centromere on long arm of human chromosome 21; (3) In 1524 metaphases scored, about 99. 95% of hybridization signals were located on chromosome 21; (4) The signals were very bright and recognizable easily in both metaphases and interphases nuclei; (5) The expected copy number of chromosome 21 was detected by FISH with the probe in more than 98.40% of interphase nuclei and 99.60% of metaphases. Conclusion The DNA probe reported here is suitable for the detection of chromosome 21 copy number in interphase nuclei and for the study of segregation of chromosome 21 during mitosis of human lymphocytes in culture.

Original languageEnglish (US)
Pages (from-to)36-40
Number of pages5
JournalChinese Journal of Medical Genetics
Volume16
Issue number1
StatePublished - 1999
Externally publishedYes

Fingerprint

Chromosomes, Human, Pair 21
Interphase
DNA Probes
Chromosomes
Fluorescence In Situ Hybridization
DNA
Fluorescence
Metaphase
Lymphocytes
Chromosomes, Human, Pair 4
Chromosomes, Human, Pair 3
Centromere
Human Chromosomes
Down Syndrome
Cell culture
Mitosis
Clone Cells
Polymerase Chain Reaction

Keywords

  • Chromosome 21
  • DNA probe
  • Fluorescence in situ hybridization
  • Interphase nucleus
  • Yeast artificial chromosome

ASJC Scopus subject areas

  • Genetics(clinical)
  • Bioengineering

Cite this

A DNA probe suitable for the detection of chromosome 21 copy number in human interphase nuclei by fluorescence in situ hybridization. / Shi, Q.; Shan, X.; Zhang, J.; Zhang, X.; Cheng, Y.; Dang, X.; Huang, Haojie; Yu, L.; Zhao, S.; Zheng, Q.; Id, A.

In: Chinese Journal of Medical Genetics, Vol. 16, No. 1, 1999, p. 36-40.

Research output: Contribution to journalArticle

Shi, Q, Shan, X, Zhang, J, Zhang, X, Cheng, Y, Dang, X, Huang, H, Yu, L, Zhao, S, Zheng, Q & Id, A 1999, 'A DNA probe suitable for the detection of chromosome 21 copy number in human interphase nuclei by fluorescence in situ hybridization', Chinese Journal of Medical Genetics, vol. 16, no. 1, pp. 36-40.
Shi, Q. ; Shan, X. ; Zhang, J. ; Zhang, X. ; Cheng, Y. ; Dang, X. ; Huang, Haojie ; Yu, L. ; Zhao, S. ; Zheng, Q. ; Id, A. / A DNA probe suitable for the detection of chromosome 21 copy number in human interphase nuclei by fluorescence in situ hybridization. In: Chinese Journal of Medical Genetics. 1999 ; Vol. 16, No. 1. pp. 36-40.
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AU - Shi, Q.

AU - Shan, X.

AU - Zhang, J.

AU - Zhang, X.

AU - Cheng, Y.

AU - Dang, X.

AU - Huang, Haojie

AU - Yu, L.

AU - Zhao, S.

AU - Zheng, Q.

AU - Id, A.

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N2 - Objective To prepare DNA probe which can be used to accurately detect chromosome 21 copy number in human interphase nuclei by fluorescence in situ hybridization (FISH). Methods A probe was produced by universal primer PCR from YAC clone 881D2 and characterized by FISH to metaphases and interphase nuclei of peripheral lymphocytes from 8 normal individuals and 5 patients suffering from trisomy 21. Results A DNA probe was produced and its characteristics were: (1) Most of the probes ranged in size from 350bp to 750bp; (2) The hybridization signals of the probes were located near centromere on long arm of human chromosome 21; (3) In 1524 metaphases scored, about 99. 95% of hybridization signals were located on chromosome 21; (4) The signals were very bright and recognizable easily in both metaphases and interphases nuclei; (5) The expected copy number of chromosome 21 was detected by FISH with the probe in more than 98.40% of interphase nuclei and 99.60% of metaphases. Conclusion The DNA probe reported here is suitable for the detection of chromosome 21 copy number in interphase nuclei and for the study of segregation of chromosome 21 during mitosis of human lymphocytes in culture.

AB - Objective To prepare DNA probe which can be used to accurately detect chromosome 21 copy number in human interphase nuclei by fluorescence in situ hybridization (FISH). Methods A probe was produced by universal primer PCR from YAC clone 881D2 and characterized by FISH to metaphases and interphase nuclei of peripheral lymphocytes from 8 normal individuals and 5 patients suffering from trisomy 21. Results A DNA probe was produced and its characteristics were: (1) Most of the probes ranged in size from 350bp to 750bp; (2) The hybridization signals of the probes were located near centromere on long arm of human chromosome 21; (3) In 1524 metaphases scored, about 99. 95% of hybridization signals were located on chromosome 21; (4) The signals were very bright and recognizable easily in both metaphases and interphases nuclei; (5) The expected copy number of chromosome 21 was detected by FISH with the probe in more than 98.40% of interphase nuclei and 99.60% of metaphases. Conclusion The DNA probe reported here is suitable for the detection of chromosome 21 copy number in interphase nuclei and for the study of segregation of chromosome 21 during mitosis of human lymphocytes in culture.

KW - Chromosome 21

KW - DNA probe

KW - Fluorescence in situ hybridization

KW - Interphase nucleus

KW - Yeast artificial chromosome

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