A disposable tear glucose biosensor - Part 1: Design and concept testing

Daniel K. Bishop, Jeffrey T. La Belle, Stephen R. Vossler, Dharmendra R. Patel, Curtiss B. Cook

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Background: Tear glucose has been suggested previously as a potential approach for the noninvasive estimation of blood glucose. While the topic remains unresolved, an overview of previous studies suggests the importance of a tear sampling approach and warrants new technology development. A concept device is presented that meets the needs of a tear glucose biosensor. Methods: Three approaches to chronoamperometric glucose sensing were evaluated, including glucose oxidase mediated by potassium ferricyanide or oxygen with a hydrogen peroxide catalyst, Prussian blue, and potassium ferricyanidemediated glucose dehydrogenase. For tear sampling, calcium alginate, poly(2-hydroxyethyl methacrylate), and polyurethane foam were screened as an absorbent tear sampling material. A quantitative model based on the proposed function of concept device was created. Results: For glucose sensing, it was found that potassium ferricyanide with glucose dehydrogenase was ideal, featuring oxygen insensitivity, long-term stability, and a lower limit of detection of 2 μM glucose. Polyurethane foam possessed all of the required characteristics for tear sampling, including reproducible sampling from a hydrogel-simulated, eye surface (4.2 ± 0.5 μl; n = 8). It is estimated that 100 μM of glucose tear fluid would yield 135 nA (14.9% relative standard deviation). Conclusion: A novel concept device for tear glucose sampling was presented, and the key functions of this device were tested and used to model the performance of the final device. Based on these promising initial results, the device is achievable and within reach of current technical capabilities, setting the stage for prototype development.

Original languageEnglish (US)
Pages (from-to)299-306
Number of pages8
JournalJournal of diabetes science and technology
Volume4
Issue number2
StatePublished - Mar 2010

Fingerprint

Biosensing Techniques
Tears
Biosensors
Glucose
Sampling
Testing
Equipment and Supplies
Glucose 1-Dehydrogenase
Potassium
Oxygen
Polyurethanes
Glucose Oxidase
Foams
Hydrogel
Hydrogen Peroxide
Blood Glucose
Glucose oxidase
Alginate
Limit of Detection
Hydrogels

Keywords

  • Biosensor
  • Diabetes mellitus
  • Glucose monitoring
  • Tear glucose monitoring

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Internal Medicine
  • Bioengineering
  • Biomedical Engineering

Cite this

Bishop, D. K., La Belle, J. T., Vossler, S. R., Patel, D. R., & Cook, C. B. (2010). A disposable tear glucose biosensor - Part 1: Design and concept testing. Journal of diabetes science and technology, 4(2), 299-306.

A disposable tear glucose biosensor - Part 1 : Design and concept testing. / Bishop, Daniel K.; La Belle, Jeffrey T.; Vossler, Stephen R.; Patel, Dharmendra R.; Cook, Curtiss B.

In: Journal of diabetes science and technology, Vol. 4, No. 2, 03.2010, p. 299-306.

Research output: Contribution to journalArticle

Bishop, DK, La Belle, JT, Vossler, SR, Patel, DR & Cook, CB 2010, 'A disposable tear glucose biosensor - Part 1: Design and concept testing', Journal of diabetes science and technology, vol. 4, no. 2, pp. 299-306.
Bishop DK, La Belle JT, Vossler SR, Patel DR, Cook CB. A disposable tear glucose biosensor - Part 1: Design and concept testing. Journal of diabetes science and technology. 2010 Mar;4(2):299-306.
Bishop, Daniel K. ; La Belle, Jeffrey T. ; Vossler, Stephen R. ; Patel, Dharmendra R. ; Cook, Curtiss B. / A disposable tear glucose biosensor - Part 1 : Design and concept testing. In: Journal of diabetes science and technology. 2010 ; Vol. 4, No. 2. pp. 299-306.
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