A direct mechanical method for accurate and efficient adenoviral vector delivery to tissues

V. G. Khurana, D. A. Weiler, T. A. Witt, L. A. Smith, L. S. Kleppe, J. E. Parisi, R. D. Simari, T. O'Brien, S. J. Russell, Z. S. Katusic

Research output: Contribution to journalReview article

12 Scopus citations

Abstract

We describe a mechanical method for delivery of adenoviral vector to the adventitial surface of arteries and to other tissues. Our goal was to characterize, principally in intact carotid artery, the morphological, biochemical, and functional effects of mechanical delivery of a recombinant β-galactosidase-expressing adenoviral vector following its direct application using a small paintbrush. Our ex vivo and in vivo data demonstrate efficient, accurate, and rapid transduction of arteries without compromise of their morphological, biochemical, and functional integrity. We also demonstrate the general applicability of this technique in vivo via transduction of skeletal muscle, fibrotendinous tissue, peritoneum, serosal surface of bowel, and wounded skin. We conclude that direct mechanical delivery of an adenoviral vector to tissues using a suitable paintbrush represents an intuitive, accurate, and effective means of augmenting gene transfer efficiency, and may be a useful adjunct to other delivery methods.

Original languageEnglish (US)
Pages (from-to)443-452
Number of pages10
JournalGene Therapy
Volume10
Issue number5
DOIs
StatePublished - Mar 2003

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Keywords

  • Adenovirus
  • Carotid artery
  • Gene therapy
  • Nitric oxide
  • Paintbrush
  • β-galactosidase

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Genetics

Cite this

Khurana, V. G., Weiler, D. A., Witt, T. A., Smith, L. A., Kleppe, L. S., Parisi, J. E., Simari, R. D., O'Brien, T., Russell, S. J., & Katusic, Z. S. (2003). A direct mechanical method for accurate and efficient adenoviral vector delivery to tissues. Gene Therapy, 10(5), 443-452. https://doi.org/10.1038/sj.gt.3301907