Abstract
The dynamic immune response to various diseases and therapies has been considered a promising indicator of disease status and therapeutic effectiveness. For instance, the human peripheral blood mononuclear cell (PBMC), as a major player in the immune system, is an important index to indicate a patient’s immune function. Therefore, establishing a simple yet sensitive tool that can frequently assess the immune system during the course of disease and treatment is of great im-portance. This study introduced an integrated system that includes an electrochemical impedance spectroscope (EIS)-based biosensor in a digital microfluidic (DMF) device, to quantify the PBMC abundance with minimally trained hands. Moreover, we exploited the unique droplet manipulation feature of the DMF platform and conducted a dynamic cell capture assay, which enhanced the detection signal by 2.4-fold. This integrated system was able to detect as few as 104 PBMCs per mL, presenting suitable sensitivity to quantify PBMCs. This integrated system is easy-to-operate and sensitive, and therefore holds great potential as a powerful tool to profile immune-mediated therapeutic responses in a timely manner, which can be further evolved as a point-of-care diagnostic device to conduct near-patient tests from blood samples.
Original language | English (US) |
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Article number | 330 |
Journal | Biosensors |
Volume | 12 |
Issue number | 5 |
DOIs | |
State | Published - May 2022 |
Keywords
- cell-based immunoassay
- digital microfluidics
- interdigitated electrode
- non-faradic electrochemical impedance spectroscopy (EIS)
ASJC Scopus subject areas
- Analytical Chemistry
- Biotechnology
- Biomedical Engineering
- Instrumentation
- Engineering (miscellaneous)
- Clinical Biochemistry