A cytogenetic study of 53 human gliomas

Robert Brian Jenkins, David W. Kimmel, Cheryl A. Moertel, Cloann G. Schultz, Bernd W. Scheithauer, Patrick J. Kelly, Gordon W. Dewald

Research output: Contribution to journalArticle

222 Citations (Scopus)

Abstract

Cytogenetic studies were performed on human glioma samples obtained by stereotactic biopsy, stereotactic craniotomy, or routine craniotomy. Using in situ culture and robotic harvesting techniques, we obtained suitable metaphases in 50 (94%) of 53 tumors, including 28 diffuse astrocytomas, four juvenile pilocytic astrocytomas, two gliosarcomas, three other miscellaneous astrocytomas, eight oligodendrogliomas, four mixed oligodendroglioma-astrocytomas, and four ependymomas. Cytogenetic studies were performed only on primary cultures; the mean culture time was 9.6 days (range 1-31 days). One or more chromosomally abnormal clones were observed in 35 (66%) tumors. Eleven (21%) other specimens had random nonclonal chromosome abnormalities. In four (8%) specimens, no chromosome abnormalities were noted. The results of this study suggest that grade 3 and 4 tumors are more likely to contain an abnormal clone than tumors of grade 1 or 2 (p < 0.01). The most common numeric chromosome abnormalities were -6, +7, -10, -13, -14, -15, -18, and -Y. The most common structural abnormalities involved 1p, 6q, 7q, 8p, 9p, 11p, 11q, 13q, and 19q. Four tumors had two or more independent clones and ten contained subclones demonstrating karyotype evolution. With in situ culture and robotic harvesting techniques, cytogenetic studies can be successful on nearly all human gliomas, including those derived from small stereotactic biopsies.

Original languageEnglish (US)
Pages (from-to)253-279
Number of pages27
JournalCancer Genetics and Cytogenetics
Volume39
Issue number2
DOIs
StatePublished - 1989

Fingerprint

Glioma
Cytogenetics
Astrocytoma
Chromosome Aberrations
Oligodendroglioma
Clone Cells
Craniotomy
Robotics
Neoplasms
Gliosarcoma
Biopsy
Ependymoma
Cytogenetic Analysis
Metaphase
Karyotype

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

Cite this

Jenkins, R. B., Kimmel, D. W., Moertel, C. A., Schultz, C. G., Scheithauer, B. W., Kelly, P. J., & Dewald, G. W. (1989). A cytogenetic study of 53 human gliomas. Cancer Genetics and Cytogenetics, 39(2), 253-279. https://doi.org/10.1016/0165-4608(89)90192-1

A cytogenetic study of 53 human gliomas. / Jenkins, Robert Brian; Kimmel, David W.; Moertel, Cheryl A.; Schultz, Cloann G.; Scheithauer, Bernd W.; Kelly, Patrick J.; Dewald, Gordon W.

In: Cancer Genetics and Cytogenetics, Vol. 39, No. 2, 1989, p. 253-279.

Research output: Contribution to journalArticle

Jenkins, RB, Kimmel, DW, Moertel, CA, Schultz, CG, Scheithauer, BW, Kelly, PJ & Dewald, GW 1989, 'A cytogenetic study of 53 human gliomas', Cancer Genetics and Cytogenetics, vol. 39, no. 2, pp. 253-279. https://doi.org/10.1016/0165-4608(89)90192-1
Jenkins RB, Kimmel DW, Moertel CA, Schultz CG, Scheithauer BW, Kelly PJ et al. A cytogenetic study of 53 human gliomas. Cancer Genetics and Cytogenetics. 1989;39(2):253-279. https://doi.org/10.1016/0165-4608(89)90192-1
Jenkins, Robert Brian ; Kimmel, David W. ; Moertel, Cheryl A. ; Schultz, Cloann G. ; Scheithauer, Bernd W. ; Kelly, Patrick J. ; Dewald, Gordon W. / A cytogenetic study of 53 human gliomas. In: Cancer Genetics and Cytogenetics. 1989 ; Vol. 39, No. 2. pp. 253-279.
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