A CRISPR reimagining: New twists and turns of CRISPR beyond the genome-engineering revolution

Robert J. Plummer, Yi D Guo, Ying Peng

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Despite its explosive applications in genome engineering, CRISPR (Clustered Regularly Interspersed Short Palindromic Repeats) has been developed into a versatile tool beyond its well-known nuclease function. In this prospect article, we summarize a few exciting “off-label” applications of CRISPR including manipulating DNA sequences, visualizing chromosomal loci in living cells, and modulating transcription and chromatin structures. These novel applications will likely elevate CRISPR tools into yet another level of sophistication and diversity, leading to many more exciting cell biological discoveries.

Original languageEnglish (US)
Pages (from-to)1299-1308
Number of pages10
JournalJournal of Cellular Biochemistry
Volume119
Issue number2
DOIs
StatePublished - Feb 1 2018

Fingerprint

Genes
Genome
Chromatin
DNA sequences
Transcription
Labels
Cells

Keywords

  • CRISPR
  • designer nuclease
  • genome engineering

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

A CRISPR reimagining : New twists and turns of CRISPR beyond the genome-engineering revolution. / Plummer, Robert J.; Guo, Yi D; Peng, Ying.

In: Journal of Cellular Biochemistry, Vol. 119, No. 2, 01.02.2018, p. 1299-1308.

Research output: Contribution to journalArticle

Plummer, Robert J. ; Guo, Yi D ; Peng, Ying. / A CRISPR reimagining : New twists and turns of CRISPR beyond the genome-engineering revolution. In: Journal of Cellular Biochemistry. 2018 ; Vol. 119, No. 2. pp. 1299-1308.
@article{0cbe10a56ef1464f9cdbe2a4d152db38,
title = "A CRISPR reimagining: New twists and turns of CRISPR beyond the genome-engineering revolution",
abstract = "Despite its explosive applications in genome engineering, CRISPR (Clustered Regularly Interspersed Short Palindromic Repeats) has been developed into a versatile tool beyond its well-known nuclease function. In this prospect article, we summarize a few exciting “off-label” applications of CRISPR including manipulating DNA sequences, visualizing chromosomal loci in living cells, and modulating transcription and chromatin structures. These novel applications will likely elevate CRISPR tools into yet another level of sophistication and diversity, leading to many more exciting cell biological discoveries.",
keywords = "CRISPR, designer nuclease, genome engineering",
author = "Plummer, {Robert J.} and Guo, {Yi D} and Ying Peng",
year = "2018",
month = "2",
day = "1",
doi = "10.1002/jcb.26406",
language = "English (US)",
volume = "119",
pages = "1299--1308",
journal = "Journal of Cellular Biochemistry",
issn = "0730-2312",
publisher = "Wiley-Liss Inc.",
number = "2",

}

TY - JOUR

T1 - A CRISPR reimagining

T2 - New twists and turns of CRISPR beyond the genome-engineering revolution

AU - Plummer, Robert J.

AU - Guo, Yi D

AU - Peng, Ying

PY - 2018/2/1

Y1 - 2018/2/1

N2 - Despite its explosive applications in genome engineering, CRISPR (Clustered Regularly Interspersed Short Palindromic Repeats) has been developed into a versatile tool beyond its well-known nuclease function. In this prospect article, we summarize a few exciting “off-label” applications of CRISPR including manipulating DNA sequences, visualizing chromosomal loci in living cells, and modulating transcription and chromatin structures. These novel applications will likely elevate CRISPR tools into yet another level of sophistication and diversity, leading to many more exciting cell biological discoveries.

AB - Despite its explosive applications in genome engineering, CRISPR (Clustered Regularly Interspersed Short Palindromic Repeats) has been developed into a versatile tool beyond its well-known nuclease function. In this prospect article, we summarize a few exciting “off-label” applications of CRISPR including manipulating DNA sequences, visualizing chromosomal loci in living cells, and modulating transcription and chromatin structures. These novel applications will likely elevate CRISPR tools into yet another level of sophistication and diversity, leading to many more exciting cell biological discoveries.

KW - CRISPR

KW - designer nuclease

KW - genome engineering

UR - http://www.scopus.com/inward/record.url?scp=85038830852&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85038830852&partnerID=8YFLogxK

U2 - 10.1002/jcb.26406

DO - 10.1002/jcb.26406

M3 - Article

C2 - 28926145

AN - SCOPUS:85038830852

VL - 119

SP - 1299

EP - 1308

JO - Journal of Cellular Biochemistry

JF - Journal of Cellular Biochemistry

SN - 0730-2312

IS - 2

ER -