A CRISPR reimagining: New twists and turns of CRISPR beyond the genome-engineering revolution

Robert J. Plummer; Yi Guo; Ying Peng

doi:10.1002/jcb.26406

A CRISPR reimagining: New twists and turns of CRISPR beyond the genome-engineering revolution

Robert J. Plummer, Yi Guo, Ying Peng

Biochemistry and Molecular Biology

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Despite its explosive applications in genome engineering, CRISPR (Clustered Regularly Interspersed Short Palindromic Repeats) has been developed into a versatile tool beyond its well-known nuclease function. In this prospect article, we summarize a few exciting “off-label” applications of CRISPR including manipulating DNA sequences, visualizing chromosomal loci in living cells, and modulating transcription and chromatin structures. These novel applications will likely elevate CRISPR tools into yet another level of sophistication and diversity, leading to many more exciting cell biological discoveries.

Original language	English (US)
Pages (from-to)	1299-1308
Number of pages	10
Journal	Journal of cellular biochemistry
Volume	119
Issue number	2
DOIs	https://doi.org/10.1002/jcb.26406
State	Published - Feb 2018

Keywords

CRISPR
designer nuclease
genome engineering

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

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10.1002/jcb.26406

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abstract = "Despite its explosive applications in genome engineering, CRISPR (Clustered Regularly Interspersed Short Palindromic Repeats) has been developed into a versatile tool beyond its well-known nuclease function. In this prospect article, we summarize a few exciting “off-label” applications of CRISPR including manipulating DNA sequences, visualizing chromosomal loci in living cells, and modulating transcription and chromatin structures. These novel applications will likely elevate CRISPR tools into yet another level of sophistication and diversity, leading to many more exciting cell biological discoveries.",

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A CRISPR reimagining: New twists and turns of CRISPR beyond the genome-engineering revolution

Abstract

Keywords

ASJC Scopus subject areas

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