A Conserved Role for Atlastin GTPases in Regulating Lipid Droplet Size

Robin W. Klemm, Justin P. Norton, Ronald A. Cole, Chen S. Li, Seong H. Park, Matthew M. Crane, Liying Li, Diana Jin, Alexandra Boye-Doe, Tina Y. Liu, Yoko Shibata, Hang Lu, Tom A. Rapoport, Robert V. Farese, Craig Blackstone, Yi D Guo, Ho Yi Mak

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Lipid droplets (LDs) are the major fat storage organelles in eukaryotic cells, but how their size is regulatedis unknown. Using genetic screens in C.elegans for LD morphology defects in intestinal cells, we found that mutations in atlastin, a GTPase required for homotypic fusion of endoplasmic reticulum (ER) membranes, cause not only ER morphology defects, but also a reduction in LD size. Similar results were obtained after depletion of atlastin or expression of a dominant-negative mutant, whereas overexpression of atlastin had the opposite effect. Atlastin depletion in Drosophila fat bodies also reduced LD size and decreased triglycerides in whole animals, sensitizing them to starvation. In mammalian cells, co-overexpression of atlastin-1 and REEP1, a paralog of the ER tubule-shaping protein DP1/REEP5, generates large LDs. The effect of atlastin-1 on LD size correlates with its activity to promote membrane fusion invitro. Our results indicate that atlastin-mediated fusion of ER membranes is important for LD size regulation.

Original languageEnglish (US)
Pages (from-to)1465-1475
Number of pages11
JournalCell Reports
Volume3
Issue number5
DOIs
StatePublished - May 30 2013

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GTP Phosphohydrolases
Lipids
Endoplasmic Reticulum
Fusion reactions
Membranes
Transcription Factor DP1
Fats
Defects
Membrane Fusion
Fat Body
Eukaryotic Cells
Starvation
Lipid Droplets
Cell Size
Organelles
Drosophila
Animals
Triglycerides
Cells
Mutation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Klemm, R. W., Norton, J. P., Cole, R. A., Li, C. S., Park, S. H., Crane, M. M., ... Mak, H. Y. (2013). A Conserved Role for Atlastin GTPases in Regulating Lipid Droplet Size. Cell Reports, 3(5), 1465-1475. https://doi.org/10.1016/j.celrep.2013.04.015

A Conserved Role for Atlastin GTPases in Regulating Lipid Droplet Size. / Klemm, Robin W.; Norton, Justin P.; Cole, Ronald A.; Li, Chen S.; Park, Seong H.; Crane, Matthew M.; Li, Liying; Jin, Diana; Boye-Doe, Alexandra; Liu, Tina Y.; Shibata, Yoko; Lu, Hang; Rapoport, Tom A.; Farese, Robert V.; Blackstone, Craig; Guo, Yi D; Mak, Ho Yi.

In: Cell Reports, Vol. 3, No. 5, 30.05.2013, p. 1465-1475.

Research output: Contribution to journalArticle

Klemm, RW, Norton, JP, Cole, RA, Li, CS, Park, SH, Crane, MM, Li, L, Jin, D, Boye-Doe, A, Liu, TY, Shibata, Y, Lu, H, Rapoport, TA, Farese, RV, Blackstone, C, Guo, YD & Mak, HY 2013, 'A Conserved Role for Atlastin GTPases in Regulating Lipid Droplet Size', Cell Reports, vol. 3, no. 5, pp. 1465-1475. https://doi.org/10.1016/j.celrep.2013.04.015
Klemm RW, Norton JP, Cole RA, Li CS, Park SH, Crane MM et al. A Conserved Role for Atlastin GTPases in Regulating Lipid Droplet Size. Cell Reports. 2013 May 30;3(5):1465-1475. https://doi.org/10.1016/j.celrep.2013.04.015
Klemm, Robin W. ; Norton, Justin P. ; Cole, Ronald A. ; Li, Chen S. ; Park, Seong H. ; Crane, Matthew M. ; Li, Liying ; Jin, Diana ; Boye-Doe, Alexandra ; Liu, Tina Y. ; Shibata, Yoko ; Lu, Hang ; Rapoport, Tom A. ; Farese, Robert V. ; Blackstone, Craig ; Guo, Yi D ; Mak, Ho Yi. / A Conserved Role for Atlastin GTPases in Regulating Lipid Droplet Size. In: Cell Reports. 2013 ; Vol. 3, No. 5. pp. 1465-1475.
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AU - Crane, Matthew M.

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AU - Lu, Hang

AU - Rapoport, Tom A.

AU - Farese, Robert V.

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