A computerized image analysis method for measuring elastic tissue

Thomas J Flotte, J. M. Seddon, Y. Zhang, R. J. Glynn, K. M. Egan, E. S. Gragoudas

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

A computerized image analysis technique for quantifying area fractions of elastic tissue in light microscopic sections is presented that considers the nonuniform distribution of the elastic tissue. The method uses Verhoeff-van Gieson stained sections. The analysis is stratified vertically in three uniform layers starting at the dermo-epidermal junction and horizontally by two schemes. A standard method consists of five equally spaced measurements. The densest method uses the three areas that contain the most elastic tissue. The area fractions are determined by counting the positive and total pixels (thresholding). A validity test utilizing independent physical measurements demonstrated differences of no more than 1.7%. Reliability tests for reading the same section on different days and adjacent sections showed no significant differences (p < 0.05) between the readings. Reliability tests of sections using different stain lots and adjacent biopsy sites also did not have significant differences. This method may be particularly useful for studies in which the distribution of the material to be measured may be very uneven, such as in solar elastosis.

Original languageEnglish (US)
Pages (from-to)358-362
Number of pages5
JournalJournal of Investigative Dermatology
Volume93
Issue number3
StatePublished - 1989
Externally publishedYes

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Elastic Tissue
Image analysis
Tissue
Reading
Biopsy
Coloring Agents
Pixels
Light

ASJC Scopus subject areas

  • Dermatology

Cite this

Flotte, T. J., Seddon, J. M., Zhang, Y., Glynn, R. J., Egan, K. M., & Gragoudas, E. S. (1989). A computerized image analysis method for measuring elastic tissue. Journal of Investigative Dermatology, 93(3), 358-362.

A computerized image analysis method for measuring elastic tissue. / Flotte, Thomas J; Seddon, J. M.; Zhang, Y.; Glynn, R. J.; Egan, K. M.; Gragoudas, E. S.

In: Journal of Investigative Dermatology, Vol. 93, No. 3, 1989, p. 358-362.

Research output: Contribution to journalArticle

Flotte, TJ, Seddon, JM, Zhang, Y, Glynn, RJ, Egan, KM & Gragoudas, ES 1989, 'A computerized image analysis method for measuring elastic tissue', Journal of Investigative Dermatology, vol. 93, no. 3, pp. 358-362.
Flotte TJ, Seddon JM, Zhang Y, Glynn RJ, Egan KM, Gragoudas ES. A computerized image analysis method for measuring elastic tissue. Journal of Investigative Dermatology. 1989;93(3):358-362.
Flotte, Thomas J ; Seddon, J. M. ; Zhang, Y. ; Glynn, R. J. ; Egan, K. M. ; Gragoudas, E. S. / A computerized image analysis method for measuring elastic tissue. In: Journal of Investigative Dermatology. 1989 ; Vol. 93, No. 3. pp. 358-362.
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