A comprehensive map of the human urinary proteome

Arivusudar Marimuthu, Robert N. O'Meally, Raghothama Chaerkady, Yashwanth Subbannayya, Vishalakshi Nanjappa, Praveen Kumar, Dhanashree S. Kelkar, Sneha M. Pinto, Rakesh Sharma, Santosh Renuse, Renu Goel, Rita Christopher, Bernard Delanghe, Robert N. Cole, H. C. Harsha, Akhilesh Pandey

Research output: Contribution to journalArticle

113 Scopus citations

Abstract

The study of the human urinary proteome has the potential to offer significant insights into normal physiology as well as disease pathology. The information obtained from such studies could be applied to the diagnosis of various diseases. The high sensitivity, resolution, and mass accuracy of the latest generation of mass spectrometers provides an opportunity to accurately catalog the proteins present in human urine, including those present at low levels. To this end, we carried out a comprehensive analysis of human urinary proteome from healthy individuals using high-resolution Fourier transform mass spectrometry. Importantly, we used the Orbitrap for detecting ions in both MS (resolution 60-000) and MS/MS (resolution 15-000) modes. To increase the depth of our analysis, we characterized both unfractionated as well as lectin-enriched proteins in our experiments. In all, we identified 1823 proteins with less than 1% false discovery rate, of which 671 proteins have not previously been reported as constituents of human urine. This data set should serve as a comprehensive reference list for future studies aimed at identification and characterization of urinary biomarkers for various diseases.

Original languageEnglish (US)
Pages (from-to)2734-2743
Number of pages10
JournalJournal of Proteome Research
Volume10
Issue number6
DOIs
StatePublished - Jun 3 2011

Keywords

  • biomarkers
  • body fluids
  • glycoproteins

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

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    Marimuthu, A., O'Meally, R. N., Chaerkady, R., Subbannayya, Y., Nanjappa, V., Kumar, P., Kelkar, D. S., Pinto, S. M., Sharma, R., Renuse, S., Goel, R., Christopher, R., Delanghe, B., Cole, R. N., Harsha, H. C., & Pandey, A. (2011). A comprehensive map of the human urinary proteome. Journal of Proteome Research, 10(6), 2734-2743. https://doi.org/10.1021/pr2003038