A comprehensive analysis of breast cancer microbiota and host gene expression

Kevin J. Thompson, James N. Ingle, Xiaojia Tang, Nicholas D Chia, Patricio R. Jeraldo, Marina Walther-Antonio, Karunya K. Kandimalla, Stephen Johnson, Janet Z. Yao, Sean C. Harrington, Vera Jean Suman, Liewei M Wang, Richard M Weinshilboum, Judy C Boughey, Jean-Pierre Kocher, Heidi Nelson, Matthew Philip Goetz, Krishna R Kalari

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The inflammatory tumoral-immune response alters the physiology of the tumor microenvironment, which may attenuate genomic instability. In addition to inducing inflammatory immune responses, several pathogenic bacteria produce genotoxins. However the extent of microbial contribution to the tumor microenvironment biology remains unknown. We utilized The Cancer Genome Atlas, (TCGA) breast cancer data to perform a novel experiment utilizing unmapped and mapped RNA sequencing read evidence to minimize laboratory costs and effort. Our objective was to characterize the microbiota and associate the microbiota with the tumor expression profiles, for 668 breast tumor tissues and 72 non-cancerous adjacent tissues. The prominent presence of Proteobacteria was increased in the tumor tissues and conversely Actinobacteria abundance increase in non-cancerous adjacent tissues. Further, geneset enrichment suggests Listeria spp to be associated with the expression profiles of genes involved with epithelial to mesenchymal transitions. Moreover, evidence suggests H. influenza may reside in the surrounding stromal material and was significantly associated with the proliferative pathways: G2M checkpoint, E2F transcription factors, and mitotic spindle assembly. In summary, further unraveling this complicated interplay should enable us to better diagnose and treat breast cancer patients.

Original languageEnglish (US)
Article numbere0188873
JournalPLoS One
Volume12
Issue number11
DOIs
StatePublished - Nov 1 2017

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Microbiota
Gene expression
breast neoplasms
Tumors
Breast Neoplasms
Gene Expression
gene expression
neoplasms
Tumor Microenvironment
Tissue
E2F Transcription Factors
RNA Sequence Analysis
Proteobacteria
Neoplasms
Genes
Listeria
Spindle Apparatus
Epithelial-Mesenchymal Transition
immune response
Actinobacteria

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

A comprehensive analysis of breast cancer microbiota and host gene expression. / Thompson, Kevin J.; Ingle, James N.; Tang, Xiaojia; Chia, Nicholas D; Jeraldo, Patricio R.; Walther-Antonio, Marina; Kandimalla, Karunya K.; Johnson, Stephen; Yao, Janet Z.; Harrington, Sean C.; Suman, Vera Jean; Wang, Liewei M; Weinshilboum, Richard M; Boughey, Judy C; Kocher, Jean-Pierre; Nelson, Heidi; Goetz, Matthew Philip; Kalari, Krishna R.

In: PLoS One, Vol. 12, No. 11, e0188873, 01.11.2017.

Research output: Contribution to journalArticle

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