A comparison of experimental aneurysm occlusion determination by angiography, scanning electron microscopy, MICROFIL® perfusion, and histology

Gregory M. Cruise, Elias A. Rivera, Russell M. Jones, Maria Vestal, Renu Virmani, Yong Hong Ding, David F Kallmes

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3 Scopus citations

Abstract

In clinical practice, occlusion of embolized, intracerebral aneurysms is evaluated using angiography. Standard, two-dimensional digital subtract angiography (DSA) is unable to quantify irregular aneurysm remnants, and even three-dimensional rotational angiography cannot quantify the degree of occlusion. To better understand occlusion at the aneurysm neck, we compared angiographic results with MICROFIL® perfusion, histology, and scanning electron microscopy (SEM) results in 20 elastase-induced saccular aneurysms in rabbits. Aneurysms were embolized with HydroCoil devices (n = 12) or platinum coils (n = 8). Aneurysm follow-up occurred at 2 (n = 10) and 6 (n = 10) weeks. Aneurysm occlusion was evaluated using DSA, MICROFIL perfusion, histological ground sections, and SEM. Groups were compared statistically using ANOVA and x2 tests. The MICROFIL perfusion results were not concordant with the angiographic results for the HydroCoil and platinum coil groups. Both increased and decreased occlusion was observed on the MICROFIL-perfused aneurysms when compared with angiography. The histologic occlusion results of the HydroCoil group were concordant with the angiographic results; however, unoccluded areas not visible on angiography were routinely observed on the ground sections in the platinum coil group. SEM imaging of the aneurysm neck consistently showed decreased occlusion than angiographic results for both the HydroCoil and platinum coil groups. Although histology and MICROFIL-perfusion analyses provided additional details of aneurysm occlusion when compared with angiography, complete visualization of the entire neck of the aneurysm and accurate assessment of aneurysm occlusion was possible only with SEM.

Original languageEnglish (US)
Pages (from-to)669-678
Number of pages10
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Volume91
Issue number2
DOIs
StatePublished - Nov 2009

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Keywords

  • Animal model
  • Embolization
  • Histology
  • Hydrogel
  • Platinum coils

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

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