TY - JOUR
T1 - A Compact Control System to Enable Automated Operation of Microfluidic Bioanalytical Assays
AU - Gonzalez-Suarez, Alan M.
AU - Long, Alexander
AU - Huang, Xu Hai
AU - Revzin, Alexander
N1 - Funding Information:
This work was supported in part by the grants from NIH (HD100251 and DK084567). Additional support was provided by the Center for Biomedical Discovery, the Office of Translation to Practice and Cells to Cures Strategic Initiative at Mayo Clinic.
Publisher Copyright:
© 2022 by the authors.
PY - 2022/12
Y1 - 2022/12
N2 - We describe a control system for operating valve-enabled microfluidic devices and leverage this control system to carry out a complex workflow of plasma separation from 8 μL of whole blood followed by on-chip mixing of plasma with assay reagents for biomarker detection. The control system incorporates pumps, digital pressure sensors, a microcontroller, solenoid valves and off-the-shelf components to deliver high and low air pressure in the desired temporal sequence to meter fluid flow and actuate microvalves. Importantly, our control system is portable, which is suitable for operating the microvalve-enabled microfluidic devices in the point-of-care setting.
AB - We describe a control system for operating valve-enabled microfluidic devices and leverage this control system to carry out a complex workflow of plasma separation from 8 μL of whole blood followed by on-chip mixing of plasma with assay reagents for biomarker detection. The control system incorporates pumps, digital pressure sensors, a microcontroller, solenoid valves and off-the-shelf components to deliver high and low air pressure in the desired temporal sequence to meter fluid flow and actuate microvalves. Importantly, our control system is portable, which is suitable for operating the microvalve-enabled microfluidic devices in the point-of-care setting.
KW - automation
KW - microfluidics
KW - micromechanical valves
UR - http://www.scopus.com/inward/record.url?scp=85144558440&partnerID=8YFLogxK
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U2 - 10.3390/bios12121160
DO - 10.3390/bios12121160
M3 - Article
C2 - 36551127
AN - SCOPUS:85144558440
VL - 12
JO - Biosensors
JF - Biosensors
SN - 2079-6374
IS - 12
M1 - 1160
ER -