A Compact Control System to Enable Automated Operation of Microfluidic Bioanalytical Assays

Alan M. Gonzalez-Suarez; Alexander Long; Xu Hai Huang; Alexander Revzin

doi:10.3390/bios12121160

A Compact Control System to Enable Automated Operation of Microfluidic Bioanalytical Assays

Alan M. Gonzalez-Suarez, Alexander Long, Xu Hai Huang, Alexander Revzin

Physiology & Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

We describe a control system for operating valve-enabled microfluidic devices and leverage this control system to carry out a complex workflow of plasma separation from 8 μL of whole blood followed by on-chip mixing of plasma with assay reagents for biomarker detection. The control system incorporates pumps, digital pressure sensors, a microcontroller, solenoid valves and off-the-shelf components to deliver high and low air pressure in the desired temporal sequence to meter fluid flow and actuate microvalves. Importantly, our control system is portable, which is suitable for operating the microvalve-enabled microfluidic devices in the point-of-care setting.

Original language	English (US)
Article number	1160
Journal	Biosensors
Volume	12
Issue number	12
DOIs	https://doi.org/10.3390/bios12121160
State	Published - Dec 2022

Keywords

automation
microfluidics
micromechanical valves

ASJC Scopus subject areas

Analytical Chemistry
Biotechnology
Biomedical Engineering
Instrumentation
Engineering (miscellaneous)
Clinical Biochemistry

Access to Document

10.3390/bios12121160

Cite this

@article{f569b40a9c8d40bb8675a3d62170a240,

title = "A Compact Control System to Enable Automated Operation of Microfluidic Bioanalytical Assays",

abstract = "We describe a control system for operating valve-enabled microfluidic devices and leverage this control system to carry out a complex workflow of plasma separation from 8 μL of whole blood followed by on-chip mixing of plasma with assay reagents for biomarker detection. The control system incorporates pumps, digital pressure sensors, a microcontroller, solenoid valves and off-the-shelf components to deliver high and low air pressure in the desired temporal sequence to meter fluid flow and actuate microvalves. Importantly, our control system is portable, which is suitable for operating the microvalve-enabled microfluidic devices in the point-of-care setting.",

keywords = "automation, microfluidics, micromechanical valves",

author = "Gonzalez-Suarez, {Alan M.} and Alexander Long and Huang, {Xu Hai} and Alexander Revzin",

note = "Publisher Copyright: {\textcopyright} 2022 by the authors.",

year = "2022",

month = dec,

doi = "10.3390/bios12121160",

language = "English (US)",

volume = "12",

journal = "Biosensors",

issn = "2079-6374",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "12",

}

TY - JOUR

T1 - A Compact Control System to Enable Automated Operation of Microfluidic Bioanalytical Assays

AU - Gonzalez-Suarez, Alan M.

AU - Long, Alexander

AU - Huang, Xu Hai

AU - Revzin, Alexander

PY - 2022/12

Y1 - 2022/12

N2 - We describe a control system for operating valve-enabled microfluidic devices and leverage this control system to carry out a complex workflow of plasma separation from 8 μL of whole blood followed by on-chip mixing of plasma with assay reagents for biomarker detection. The control system incorporates pumps, digital pressure sensors, a microcontroller, solenoid valves and off-the-shelf components to deliver high and low air pressure in the desired temporal sequence to meter fluid flow and actuate microvalves. Importantly, our control system is portable, which is suitable for operating the microvalve-enabled microfluidic devices in the point-of-care setting.

AB - We describe a control system for operating valve-enabled microfluidic devices and leverage this control system to carry out a complex workflow of plasma separation from 8 μL of whole blood followed by on-chip mixing of plasma with assay reagents for biomarker detection. The control system incorporates pumps, digital pressure sensors, a microcontroller, solenoid valves and off-the-shelf components to deliver high and low air pressure in the desired temporal sequence to meter fluid flow and actuate microvalves. Importantly, our control system is portable, which is suitable for operating the microvalve-enabled microfluidic devices in the point-of-care setting.

KW - automation

KW - microfluidics

KW - micromechanical valves

UR - http://www.scopus.com/inward/record.url?scp=85144558440&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85144558440&partnerID=8YFLogxK

U2 - 10.3390/bios12121160

DO - 10.3390/bios12121160

M3 - Article

C2 - 36551127

AN - SCOPUS:85144558440

SN - 2079-6374

VL - 12

JO - Biosensors

JF - Biosensors

IS - 12

M1 - 1160

ER -

A Compact Control System to Enable Automated Operation of Microfluidic Bioanalytical Assays

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this