A commensal Bifidobacterium longum strain prevents gluten-related immunopathology in mice through expression of a serine protease inhibitor

J. L. McCarville, J. Dong, A. Caminero, M. Bermudez-Brito, J. Jury, Joseph A Murray, S. Duboux, M. Steinmann, M. Delley, M. Tangyu, P. Langella, A. Mercenier, G. Bergonzelli, E. F. Verdu

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Microbiota-modulating strategies, including probiotic administration, have been tested for the treatment of chronic gastrointestinal diseases despite limited information regarding their mechanisms of action. We previously demonstrated that patients with active celiac disease have decreased duodenal expression of elafin, a human serine protease inhibitor, and supplementation of elafin by a recombinant Lactococcus lactis strain prevents gliadin-induced immunopathology in the NOD/DQ8 mouse model of gluten sensitivity. The commensal probiotic strain Bifidobacterium longum NCC2705 produces a serine protease inhibitor (Srp) that exhibits immune-modulating properties. Here, we demonstrate that B. longum NCC2705, but not a srp knockout mutant, attenuates gliadin-induced immunopathology and impacts intestinal microbial composition in NOD/DQ8 mice. Our results highlight the beneficial effects of a serine protease inhibitor produced by commensal B. longum strains.

Original languageEnglish (US)
Article numbere01323-17
JournalApplied and Environmental Microbiology
Volume83
Issue number19
DOIs
StatePublished - Jan 1 2017
Externally publishedYes

Fingerprint

immunopathology
Bifidobacterium longum
Serine Proteinase Inhibitors
commensal
Glutens
Elafin
serine proteinases
proteinase inhibitors
gluten
Gliadin
inhibitor
Inbred NOD Mouse
probiotics
gliadin
Probiotics
mice
Lactococcus lactis
knockout mutants
celiac disease
Gastrointestinal Diseases

Keywords

  • Celiac
  • Commensal
  • Gluten
  • Microbiota
  • Probiotic
  • Serpin

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

A commensal Bifidobacterium longum strain prevents gluten-related immunopathology in mice through expression of a serine protease inhibitor. / McCarville, J. L.; Dong, J.; Caminero, A.; Bermudez-Brito, M.; Jury, J.; Murray, Joseph A; Duboux, S.; Steinmann, M.; Delley, M.; Tangyu, M.; Langella, P.; Mercenier, A.; Bergonzelli, G.; Verdu, E. F.

In: Applied and Environmental Microbiology, Vol. 83, No. 19, e01323-17, 01.01.2017.

Research output: Contribution to journalArticle

McCarville, JL, Dong, J, Caminero, A, Bermudez-Brito, M, Jury, J, Murray, JA, Duboux, S, Steinmann, M, Delley, M, Tangyu, M, Langella, P, Mercenier, A, Bergonzelli, G & Verdu, EF 2017, 'A commensal Bifidobacterium longum strain prevents gluten-related immunopathology in mice through expression of a serine protease inhibitor', Applied and Environmental Microbiology, vol. 83, no. 19, e01323-17. https://doi.org/10.1128/AEM.01323-17
McCarville, J. L. ; Dong, J. ; Caminero, A. ; Bermudez-Brito, M. ; Jury, J. ; Murray, Joseph A ; Duboux, S. ; Steinmann, M. ; Delley, M. ; Tangyu, M. ; Langella, P. ; Mercenier, A. ; Bergonzelli, G. ; Verdu, E. F. / A commensal Bifidobacterium longum strain prevents gluten-related immunopathology in mice through expression of a serine protease inhibitor. In: Applied and Environmental Microbiology. 2017 ; Vol. 83, No. 19.
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