A ATPase cycle of the mitotic motor CENP-E

Steven Rosenfeld, Marilyn van Duffelen, William M. Behnke-Parks, Christopher Beadle, John Correia, Jun Xing

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

We have previously shown that the mitotic motor centrosome protein E (CENP-E) is capable of walking for more than 250 steps on its microtubule track without dissociating. We have examined the kinetics of this molecular motor to see if its enzymology explains this remarkable degree of processivity. We find that like the highly processive transport motor kinesin 1, the enzymatic cycle of CENP-E is characterized by rapid ATP binding, multiple enzymatic turnovers per diffusive encounter, and gating of nucleotide binding. These features endow CENP-E with a high duty cycle, a prerequisite for processivity. However, unlike kinesin 1, neck linker docking in CENP-E is slow, occurring at a rate closer to that for Eg5, a mitotic kinesin that takes only 5-10 steps per processive run. These results suggest that like kinesin 1, features outside of the catalytic domain of CENP-E may also play a role in regulating the processive behavior of this motor.

Original languageEnglish (US)
Pages (from-to)32858-32868
Number of pages11
JournalJournal of Biological Chemistry
Volume284
Issue number47
DOIs
StatePublished - Nov 20 2009
Externally publishedYes

Fingerprint

Centrosome
Kinesin
Adenosine Triphosphatases
Proteins
Microtubules
Walking
Catalytic Domain
Neck
Nucleotides
Adenosine Triphosphate
Kinetics

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Rosenfeld, S., van Duffelen, M., Behnke-Parks, W. M., Beadle, C., Correia, J., & Xing, J. (2009). A ATPase cycle of the mitotic motor CENP-E. Journal of Biological Chemistry, 284(47), 32858-32868. https://doi.org/10.1074/jbc.M109.041210

A ATPase cycle of the mitotic motor CENP-E. / Rosenfeld, Steven; van Duffelen, Marilyn; Behnke-Parks, William M.; Beadle, Christopher; Correia, John; Xing, Jun.

In: Journal of Biological Chemistry, Vol. 284, No. 47, 20.11.2009, p. 32858-32868.

Research output: Contribution to journalArticle

Rosenfeld, S, van Duffelen, M, Behnke-Parks, WM, Beadle, C, Correia, J & Xing, J 2009, 'A ATPase cycle of the mitotic motor CENP-E', Journal of Biological Chemistry, vol. 284, no. 47, pp. 32858-32868. https://doi.org/10.1074/jbc.M109.041210
Rosenfeld S, van Duffelen M, Behnke-Parks WM, Beadle C, Correia J, Xing J. A ATPase cycle of the mitotic motor CENP-E. Journal of Biological Chemistry. 2009 Nov 20;284(47):32858-32868. https://doi.org/10.1074/jbc.M109.041210
Rosenfeld, Steven ; van Duffelen, Marilyn ; Behnke-Parks, William M. ; Beadle, Christopher ; Correia, John ; Xing, Jun. / A ATPase cycle of the mitotic motor CENP-E. In: Journal of Biological Chemistry. 2009 ; Vol. 284, No. 47. pp. 32858-32868.
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