8q24 clear cell renal cell carcinoma germline variant is associated with VHL mutation status and clinical aggressiveness

Jeanette E. Eckel-Passow, Huihuang Yan, Matthew L. Kosel, Daniel Serie, Paul A. Decker, Robert B. Jenkins, Brian Costello, Bradley Leibovich, Thai H. Ho, Alexander Parker

Research output: Contribution to journalArticlepeer-review

Abstract

Background: The four most commonly-mutated genes in clear cell renal cell carcinoma (ccRCC) tumors are BAP1, PBRM1, SETD2 and VHL. And, there are currently 14 known RCC germline variants that have been reproducibly shown to be associated with RCC risk. However, the association of germline genetics with tumor genetics and clinical aggressiveness are unknown. Methods: We analyzed 420 ccRCC patients from The Cancer Genome Atlas. Molecular subtype was determined based on acquired mutations in BAP1, PBRM1, SETD2 and VHL. Aggressive subtype was defined clinically using Mayo SSIGN score and molecularly using the ccA/ccB gene expression subtype. Publically-available Hi-C data were used to link germline risk variants with candidate target genes. Results: The 8q24 variant rs35252396 was significantly associated with VHL mutation status (OR = 1.6, p = 0.0037) and SSIGN score (OR = 1.9, p = 0.00094), after adjusting for multiple comparisons. We observed that, while some germline variants have interactions with nearby genes, some variants demonstrate long-range interactions with target genes. Conclusions: These data further demonstrate the link between rs35252396, HIF pathway and ccRCC clinical aggressiveness, providing a more comprehensive picture of how germline genetics and tumor genetics interact with respect to tumor development and progression.

Original languageEnglish (US)
Article number173
JournalBMC Urology
Volume20
Issue number1
DOIs
StatePublished - Dec 1 2020

Keywords

  • GWAS
  • Hi-C
  • Kidney
  • Subtype

ASJC Scopus subject areas

  • Reproductive Medicine
  • Urology

Fingerprint Dive into the research topics of '8q24 clear cell renal cell carcinoma germline variant is associated with VHL mutation status and clinical aggressiveness'. Together they form a unique fingerprint.

Cite this