5-hydroxytryptamine depolarizes neurons of cat pancreatic ganglia

R. C. Ma, J. H. Szurszewski

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Pancreatic ganglia contain 5-hydroxytryptamine (5-HT)-immunoreactive axons, some of which are extensions of myenteric neurons located in the pyloric antrum and proximal duodenum. The present study investigated the effect of 5-HT on the membrane potential of cat pancreatic ganglion neurons by means of intracellular recordings in vitro. Pressure application of 5-HT evoked a fast depolarization in 29 of 147 neurons and a slow depolarization in 89 of 147 neurons. A biphasic response was observed in 10 of 108 neurons. The 5-HT-induced slow depolarizing response was not altered in a low Ca2+ (0.1 mM), high Mg2+ (15 mM) solution nor by hexamethonium (10-4 M) or atropine (10-6 M). The fast depolarizing response was associated with a decrease of membrane input resistance (-17.2%). The slow depolarizing response was associated with either a decrease (-19.6%) in 24, an increase (+ 25.0%) in 20, or without a detectable change of membrane input resistance in 10 out of 54 neurons tested. Conditioning hyperpolarization increased the amplitude of both fast and slow depolarizing responses. A low Na+ (68.5 mM) solution and a high K+ (23.5 mM) solution significantly reduced the amplitude of the slow depolarizing response. A low Cl- (9.6 mM) solution had no significant effect on the slow depolarization. The 5-HT3 receptor antagonist MDL 72222 (Bemesetron) blocked the 5-HT-evoked fast depolarizing response. BRL 24924 (Renzapride) and 5 HT-DP, antagonists for the putative 5-HT1P receptor, blocked the slow depolarizing response. The 5-HT3 receptor agonist 2-methyl-5-HT evoked a fast depolarizing response and MCPP, an agonist for the putative 5-HT1P receptor, evoked a slow depolarizing response. Spiperone (a 5-HT1A receptor antagonist) and mianserin (a 5-HT2 receptor antagonist) had no effect on either depolarizing response to 5-HT. The results show that pancreatic ganglion neurons responded to 5-HT with fast and slow depolarizing responses. The data suggest that these responses were mediated by the 5-HT3 receptor and the putative 5-HT1P receptor, respectively.

Original languageEnglish (US)
Pages (from-to)78-86
Number of pages9
JournalJournal of the Autonomic Nervous System
Volume57
Issue number1-2
StatePublished - Feb 5 1996

Fingerprint

Ganglia
Serotonin
Cats
Neurons
Receptors, Serotonin, 5-HT3
Serotonin 5-HT3 Receptor Agonists
Serotonin 5-HT1 Receptor Antagonists
Mianserin
Serotonin 5-HT3 Receptor Antagonists
Serotonin 5-HT2 Receptor Antagonists
Pyloric Antrum
Spiperone
Hexamethonium
Serotonin Antagonists
Receptor, Serotonin, 5-HT1A
Membranes
Atropine
Duodenum
Membrane Potentials
Axons

Keywords

  • 5-hydroxytryptamine
  • Cat
  • Membrane depolarization
  • Pancreas
  • Pancreatic ganglion

ASJC Scopus subject areas

  • Physiology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

5-hydroxytryptamine depolarizes neurons of cat pancreatic ganglia. / Ma, R. C.; Szurszewski, J. H.

In: Journal of the Autonomic Nervous System, Vol. 57, No. 1-2, 05.02.1996, p. 78-86.

Research output: Contribution to journalArticle

Ma, R. C. ; Szurszewski, J. H. / 5-hydroxytryptamine depolarizes neurons of cat pancreatic ganglia. In: Journal of the Autonomic Nervous System. 1996 ; Vol. 57, No. 1-2. pp. 78-86.
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