4-Hydroxytamoxifen, an active metabolite of tamoxifen, does not alter the radiation sensitivity of MCF-7 breast carcinoma cells irradiated in vitro

Jann N Sarkaria, Elizabeth M. Miller, Christopher J. Parker, V. Craig Jordan, R. Timothy Mulcahy

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

The effect of 4-hydroxytamoxifen (4OH-TAM), the potent anti-estrogenic metabolite of tamoxifen, on the radiosensitivity of MCF-7 cells irradiated in vitro was determined. Radiation dose response curves were generated for MCF-7 cells maintained and irradiated in phenol red-free medium containing 10-10 M estradiol (E2) with or without 10-7 M 4OH-TAM. Immediately after irradiation cells were transferred to medium containing 10-10 M E2 supplemented with bovine serum to stimulate colony formation. Estradiol-stimulated cell proliferation was inhibited by 10-7 M 4OH-TAM, but radiation sensitivity was not significantly altered (p > 0.3). Continued incubation in the absence of E2 for an additional 24 hours after irradiation likewise failed to alter the radiosensitivity of 4OH-TAM-treated MCF-7 cells. These studies indicate that growth-inhibitory concentrations of the anti-estrogen 4OH-TAM do not modify the in vitro radiation sensitivity of this line of human breast carcinoma cells.

Original languageEnglish (US)
Pages (from-to)159-165
Number of pages7
JournalBreast Cancer Research and Treatment
Volume30
Issue number2
DOIs
StatePublished - Jan 1994
Externally publishedYes

Fingerprint

Radiation Tolerance
Tamoxifen
MCF-7 Cells
Breast Neoplasms
Estradiol
Phenolsulfonphthalein
Estrogens
Cell Proliferation
Radiation
afimoxifene
In Vitro Techniques
Growth
Serum

Keywords

  • 4-hydroxytamoxifen
  • growth inhibition
  • MCF-7 cells
  • radiation sensitivity
  • tamoxifen

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

4-Hydroxytamoxifen, an active metabolite of tamoxifen, does not alter the radiation sensitivity of MCF-7 breast carcinoma cells irradiated in vitro. / Sarkaria, Jann N; Miller, Elizabeth M.; Parker, Christopher J.; Jordan, V. Craig; Mulcahy, R. Timothy.

In: Breast Cancer Research and Treatment, Vol. 30, No. 2, 01.1994, p. 159-165.

Research output: Contribution to journalArticle

Sarkaria, Jann N ; Miller, Elizabeth M. ; Parker, Christopher J. ; Jordan, V. Craig ; Mulcahy, R. Timothy. / 4-Hydroxytamoxifen, an active metabolite of tamoxifen, does not alter the radiation sensitivity of MCF-7 breast carcinoma cells irradiated in vitro. In: Breast Cancer Research and Treatment. 1994 ; Vol. 30, No. 2. pp. 159-165.
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abstract = "The effect of 4-hydroxytamoxifen (4OH-TAM), the potent anti-estrogenic metabolite of tamoxifen, on the radiosensitivity of MCF-7 cells irradiated in vitro was determined. Radiation dose response curves were generated for MCF-7 cells maintained and irradiated in phenol red-free medium containing 10-10 M estradiol (E2) with or without 10-7 M 4OH-TAM. Immediately after irradiation cells were transferred to medium containing 10-10 M E2 supplemented with bovine serum to stimulate colony formation. Estradiol-stimulated cell proliferation was inhibited by 10-7 M 4OH-TAM, but radiation sensitivity was not significantly altered (p > 0.3). Continued incubation in the absence of E2 for an additional 24 hours after irradiation likewise failed to alter the radiosensitivity of 4OH-TAM-treated MCF-7 cells. These studies indicate that growth-inhibitory concentrations of the anti-estrogen 4OH-TAM do not modify the in vitro radiation sensitivity of this line of human breast carcinoma cells.",
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