3D printing for bio-synthetic biliary stents

Christen J. Boyer, Moheb Boktor, Hrishikesh Samant, Luke A. White, Yuping Wang, David H. Ballard, Robert C Huebert, Jennifer E. Woerner, Ghali E. Ghali, Jonathan S. Alexander

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Three-dimensional (3D) printing is an additive manufacturing method that holds great potential in a variety of future patient-specific medical technologies. This project validated a novel crosslinked polyvinyl alcohol (XL-PVA) 3D printed stent infused with collagen, human placental mesenchymal stem cells (PMSCs), and cholangiocytes. The biofabrication method in the present study examined 3D printing and collagen injection molding for rapid prototyping of customized living biliary stents with clinical applications in the setting of malignant and benign bile duct obstructions. XL-PVA stents showed hydrophilic swelling and addition of radiocontrast to the stent matrix improved radiographic opacity. Collagen loaded with PMSCs contracted tightly around hydrophilic stents and dense choloangiocyte coatings were verified through histology and fluorescence microscopy. It is anticipated that design elements used in these stents may enable appropriate stent placement, provide protection of the stent-stem cell matrix against bile constituents, and potentially limit biofilm development. Overall, this approach may allow physicians to create personalized bio-integrating stents for use in biliary procedures and lays a foundation for new patient-specific stent fabrication techniques.

Original languageEnglish (US)
Article number16
JournalBioengineering
Volume6
Issue number1
DOIs
StatePublished - Jan 1 2019

Fingerprint

Stents
Printing
Stem cells
Collagen
3D printers
Polyvinyl Alcohol
Histology
Fluorescence microscopy
Polyvinyl alcohols
Opacity
Biofilms
Rapid prototyping
Injection molding
Ducts
Swelling
Fabrication
Coatings

Keywords

  • 3D printing
  • Hepatobiliary stent
  • Medical device
  • Personalized medicine
  • Stem cells
  • Tissue engineering

ASJC Scopus subject areas

  • Bioengineering

Cite this

Boyer, C. J., Boktor, M., Samant, H., White, L. A., Wang, Y., Ballard, D. H., ... Alexander, J. S. (2019). 3D printing for bio-synthetic biliary stents. Bioengineering, 6(1), [16]. https://doi.org/10.3390/bioengineering6010016

3D printing for bio-synthetic biliary stents. / Boyer, Christen J.; Boktor, Moheb; Samant, Hrishikesh; White, Luke A.; Wang, Yuping; Ballard, David H.; Huebert, Robert C; Woerner, Jennifer E.; Ghali, Ghali E.; Alexander, Jonathan S.

In: Bioengineering, Vol. 6, No. 1, 16, 01.01.2019.

Research output: Contribution to journalArticle

Boyer, CJ, Boktor, M, Samant, H, White, LA, Wang, Y, Ballard, DH, Huebert, RC, Woerner, JE, Ghali, GE & Alexander, JS 2019, '3D printing for bio-synthetic biliary stents', Bioengineering, vol. 6, no. 1, 16. https://doi.org/10.3390/bioengineering6010016
Boyer CJ, Boktor M, Samant H, White LA, Wang Y, Ballard DH et al. 3D printing for bio-synthetic biliary stents. Bioengineering. 2019 Jan 1;6(1). 16. https://doi.org/10.3390/bioengineering6010016
Boyer, Christen J. ; Boktor, Moheb ; Samant, Hrishikesh ; White, Luke A. ; Wang, Yuping ; Ballard, David H. ; Huebert, Robert C ; Woerner, Jennifer E. ; Ghali, Ghali E. ; Alexander, Jonathan S. / 3D printing for bio-synthetic biliary stents. In: Bioengineering. 2019 ; Vol. 6, No. 1.
@article{afd1b9fcf6c741b5bc200f1775ef469b,
title = "3D printing for bio-synthetic biliary stents",
abstract = "Three-dimensional (3D) printing is an additive manufacturing method that holds great potential in a variety of future patient-specific medical technologies. This project validated a novel crosslinked polyvinyl alcohol (XL-PVA) 3D printed stent infused with collagen, human placental mesenchymal stem cells (PMSCs), and cholangiocytes. The biofabrication method in the present study examined 3D printing and collagen injection molding for rapid prototyping of customized living biliary stents with clinical applications in the setting of malignant and benign bile duct obstructions. XL-PVA stents showed hydrophilic swelling and addition of radiocontrast to the stent matrix improved radiographic opacity. Collagen loaded with PMSCs contracted tightly around hydrophilic stents and dense choloangiocyte coatings were verified through histology and fluorescence microscopy. It is anticipated that design elements used in these stents may enable appropriate stent placement, provide protection of the stent-stem cell matrix against bile constituents, and potentially limit biofilm development. Overall, this approach may allow physicians to create personalized bio-integrating stents for use in biliary procedures and lays a foundation for new patient-specific stent fabrication techniques.",
keywords = "3D printing, Hepatobiliary stent, Medical device, Personalized medicine, Stem cells, Tissue engineering",
author = "Boyer, {Christen J.} and Moheb Boktor and Hrishikesh Samant and White, {Luke A.} and Yuping Wang and Ballard, {David H.} and Huebert, {Robert C} and Woerner, {Jennifer E.} and Ghali, {Ghali E.} and Alexander, {Jonathan S.}",
year = "2019",
month = "1",
day = "1",
doi = "10.3390/bioengineering6010016",
language = "English (US)",
volume = "6",
journal = "Bioengineering",
issn = "2306-5354",
publisher = "MDPI AG",
number = "1",

}

TY - JOUR

T1 - 3D printing for bio-synthetic biliary stents

AU - Boyer, Christen J.

AU - Boktor, Moheb

AU - Samant, Hrishikesh

AU - White, Luke A.

AU - Wang, Yuping

AU - Ballard, David H.

AU - Huebert, Robert C

AU - Woerner, Jennifer E.

AU - Ghali, Ghali E.

AU - Alexander, Jonathan S.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Three-dimensional (3D) printing is an additive manufacturing method that holds great potential in a variety of future patient-specific medical technologies. This project validated a novel crosslinked polyvinyl alcohol (XL-PVA) 3D printed stent infused with collagen, human placental mesenchymal stem cells (PMSCs), and cholangiocytes. The biofabrication method in the present study examined 3D printing and collagen injection molding for rapid prototyping of customized living biliary stents with clinical applications in the setting of malignant and benign bile duct obstructions. XL-PVA stents showed hydrophilic swelling and addition of radiocontrast to the stent matrix improved radiographic opacity. Collagen loaded with PMSCs contracted tightly around hydrophilic stents and dense choloangiocyte coatings were verified through histology and fluorescence microscopy. It is anticipated that design elements used in these stents may enable appropriate stent placement, provide protection of the stent-stem cell matrix against bile constituents, and potentially limit biofilm development. Overall, this approach may allow physicians to create personalized bio-integrating stents for use in biliary procedures and lays a foundation for new patient-specific stent fabrication techniques.

AB - Three-dimensional (3D) printing is an additive manufacturing method that holds great potential in a variety of future patient-specific medical technologies. This project validated a novel crosslinked polyvinyl alcohol (XL-PVA) 3D printed stent infused with collagen, human placental mesenchymal stem cells (PMSCs), and cholangiocytes. The biofabrication method in the present study examined 3D printing and collagen injection molding for rapid prototyping of customized living biliary stents with clinical applications in the setting of malignant and benign bile duct obstructions. XL-PVA stents showed hydrophilic swelling and addition of radiocontrast to the stent matrix improved radiographic opacity. Collagen loaded with PMSCs contracted tightly around hydrophilic stents and dense choloangiocyte coatings were verified through histology and fluorescence microscopy. It is anticipated that design elements used in these stents may enable appropriate stent placement, provide protection of the stent-stem cell matrix against bile constituents, and potentially limit biofilm development. Overall, this approach may allow physicians to create personalized bio-integrating stents for use in biliary procedures and lays a foundation for new patient-specific stent fabrication techniques.

KW - 3D printing

KW - Hepatobiliary stent

KW - Medical device

KW - Personalized medicine

KW - Stem cells

KW - Tissue engineering

UR - http://www.scopus.com/inward/record.url?scp=85062215578&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85062215578&partnerID=8YFLogxK

U2 - 10.3390/bioengineering6010016

DO - 10.3390/bioengineering6010016

M3 - Article

VL - 6

JO - Bioengineering

JF - Bioengineering

SN - 2306-5354

IS - 1

M1 - 16

ER -