Abstract
Three-dimensional (3D) printing is an additive manufacturing method that holds great potential in a variety of future patient-specific medical technologies. This project validated a novel crosslinked polyvinyl alcohol (XL-PVA) 3D printed stent infused with collagen, human placental mesenchymal stem cells (PMSCs), and cholangiocytes. The biofabrication method in the present study examined 3D printing and collagen injection molding for rapid prototyping of customized living biliary stents with clinical applications in the setting of malignant and benign bile duct obstructions. XL-PVA stents showed hydrophilic swelling and addition of radiocontrast to the stent matrix improved radiographic opacity. Collagen loaded with PMSCs contracted tightly around hydrophilic stents and dense choloangiocyte coatings were verified through histology and fluorescence microscopy. It is anticipated that design elements used in these stents may enable appropriate stent placement, provide protection of the stent-stem cell matrix against bile constituents, and potentially limit biofilm development. Overall, this approach may allow physicians to create personalized bio-integrating stents for use in biliary procedures and lays a foundation for new patient-specific stent fabrication techniques.
| Original language | English (US) |
|---|---|
| Article number | 16 |
| Journal | Bioengineering |
| Volume | 6 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 2019 |
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Keywords
- 3D printing
- Hepatobiliary stent
- Medical device
- Personalized medicine
- Stem cells
- Tissue engineering
ASJC Scopus subject areas
- Bioengineering
Cite this
3D printing for bio-synthetic biliary stents. / Boyer, Christen J.; Boktor, Moheb; Samant, Hrishikesh; White, Luke A.; Wang, Yuping; Ballard, David H.; Huebert, Robert C; Woerner, Jennifer E.; Ghali, Ghali E.; Alexander, Jonathan S.
In: Bioengineering, Vol. 6, No. 1, 16, 01.01.2019.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - 3D printing for bio-synthetic biliary stents
AU - Boyer, Christen J.
AU - Boktor, Moheb
AU - Samant, Hrishikesh
AU - White, Luke A.
AU - Wang, Yuping
AU - Ballard, David H.
AU - Huebert, Robert C
AU - Woerner, Jennifer E.
AU - Ghali, Ghali E.
AU - Alexander, Jonathan S.
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Three-dimensional (3D) printing is an additive manufacturing method that holds great potential in a variety of future patient-specific medical technologies. This project validated a novel crosslinked polyvinyl alcohol (XL-PVA) 3D printed stent infused with collagen, human placental mesenchymal stem cells (PMSCs), and cholangiocytes. The biofabrication method in the present study examined 3D printing and collagen injection molding for rapid prototyping of customized living biliary stents with clinical applications in the setting of malignant and benign bile duct obstructions. XL-PVA stents showed hydrophilic swelling and addition of radiocontrast to the stent matrix improved radiographic opacity. Collagen loaded with PMSCs contracted tightly around hydrophilic stents and dense choloangiocyte coatings were verified through histology and fluorescence microscopy. It is anticipated that design elements used in these stents may enable appropriate stent placement, provide protection of the stent-stem cell matrix against bile constituents, and potentially limit biofilm development. Overall, this approach may allow physicians to create personalized bio-integrating stents for use in biliary procedures and lays a foundation for new patient-specific stent fabrication techniques.
AB - Three-dimensional (3D) printing is an additive manufacturing method that holds great potential in a variety of future patient-specific medical technologies. This project validated a novel crosslinked polyvinyl alcohol (XL-PVA) 3D printed stent infused with collagen, human placental mesenchymal stem cells (PMSCs), and cholangiocytes. The biofabrication method in the present study examined 3D printing and collagen injection molding for rapid prototyping of customized living biliary stents with clinical applications in the setting of malignant and benign bile duct obstructions. XL-PVA stents showed hydrophilic swelling and addition of radiocontrast to the stent matrix improved radiographic opacity. Collagen loaded with PMSCs contracted tightly around hydrophilic stents and dense choloangiocyte coatings were verified through histology and fluorescence microscopy. It is anticipated that design elements used in these stents may enable appropriate stent placement, provide protection of the stent-stem cell matrix against bile constituents, and potentially limit biofilm development. Overall, this approach may allow physicians to create personalized bio-integrating stents for use in biliary procedures and lays a foundation for new patient-specific stent fabrication techniques.
KW - 3D printing
KW - Hepatobiliary stent
KW - Medical device
KW - Personalized medicine
KW - Stem cells
KW - Tissue engineering
UR - http://www.scopus.com/inward/record.url?scp=85062215578&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85062215578&partnerID=8YFLogxK
U2 - 10.3390/bioengineering6010016
DO - 10.3390/bioengineering6010016
M3 - Article
AN - SCOPUS:85062215578
VL - 6
JO - Bioengineering
JF - Bioengineering
SN - 2306-5354
IS - 1
M1 - 16
ER -