3D printing for bio-synthetic biliary stents

Christen J. Boyer; Moheb Boktor; Hrishikesh Samant; Luke A. White; Yuping Wang; David H. Ballard; Robert C. Huebert; Jennifer E. Woerner; Ghali E. Ghali; Jonathan S. Alexander

doi:10.3390/bioengineering6010016

3D printing for bio-synthetic biliary stents

Christen J. Boyer, Moheb Boktor, Hrishikesh Samant, Luke A. White, Yuping Wang, David H. Ballard, Robert C. Huebert, Jennifer E. Woerner, Ghali E. Ghali, Jonathan S. Alexander

Gastroenterology and Hepatology

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Three-dimensional (3D) printing is an additive manufacturing method that holds great potential in a variety of future patient-specific medical technologies. This project validated a novel crosslinked polyvinyl alcohol (XL-PVA) 3D printed stent infused with collagen, human placental mesenchymal stem cells (PMSCs), and cholangiocytes. The biofabrication method in the present study examined 3D printing and collagen injection molding for rapid prototyping of customized living biliary stents with clinical applications in the setting of malignant and benign bile duct obstructions. XL-PVA stents showed hydrophilic swelling and addition of radiocontrast to the stent matrix improved radiographic opacity. Collagen loaded with PMSCs contracted tightly around hydrophilic stents and dense choloangiocyte coatings were verified through histology and fluorescence microscopy. It is anticipated that design elements used in these stents may enable appropriate stent placement, provide protection of the stent-stem cell matrix against bile constituents, and potentially limit biofilm development. Overall, this approach may allow physicians to create personalized bio-integrating stents for use in biliary procedures and lays a foundation for new patient-specific stent fabrication techniques.

Original language	English (US)
Article number	16
Journal	Bioengineering
Volume	6
Issue number	1
DOIs	https://doi.org/10.3390/bioengineering6010016
State	Published - Jan 2019

Keywords

3D printing
Hepatobiliary stent
Medical device
Personalized medicine
Stem cells
Tissue engineering

ASJC Scopus subject areas

Bioengineering

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title = "3D printing for bio-synthetic biliary stents",

abstract = "Three-dimensional (3D) printing is an additive manufacturing method that holds great potential in a variety of future patient-specific medical technologies. This project validated a novel crosslinked polyvinyl alcohol (XL-PVA) 3D printed stent infused with collagen, human placental mesenchymal stem cells (PMSCs), and cholangiocytes. The biofabrication method in the present study examined 3D printing and collagen injection molding for rapid prototyping of customized living biliary stents with clinical applications in the setting of malignant and benign bile duct obstructions. XL-PVA stents showed hydrophilic swelling and addition of radiocontrast to the stent matrix improved radiographic opacity. Collagen loaded with PMSCs contracted tightly around hydrophilic stents and dense choloangiocyte coatings were verified through histology and fluorescence microscopy. It is anticipated that design elements used in these stents may enable appropriate stent placement, provide protection of the stent-stem cell matrix against bile constituents, and potentially limit biofilm development. Overall, this approach may allow physicians to create personalized bio-integrating stents for use in biliary procedures and lays a foundation for new patient-specific stent fabrication techniques.",

keywords = "3D printing, Hepatobiliary stent, Medical device, Personalized medicine, Stem cells, Tissue engineering",

author = "Boyer, {Christen J.} and Moheb Boktor and Hrishikesh Samant and White, {Luke A.} and Yuping Wang and Ballard, {David H.} and Huebert, {Robert C.} and Woerner, {Jennifer E.} and Ghali, {Ghali E.} and Alexander, {Jonathan S.}",

note = "Funding Information: This work was supported by the LSU Research and Technology Foundation. (LSU LIFT2 Grant 110351233A). DHB receives salary support from National Institutes of Health TOP-TIER grant T32-EB021955. Funding Information: Funding: This work was supported by the LSU Research and Technology Foundation. (LSU LIFT2 Grant 110351233A). DHB receives salary support from National Institutes of Health TOP-TIER grant T32-EB021955. Publisher Copyright: {\textcopyright} 2019 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2019",

month = jan,

doi = "10.3390/bioengineering6010016",

language = "English (US)",

volume = "6",

journal = "Bioengineering",

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AU - Boyer, Christen J.

AU - Boktor, Moheb

AU - Samant, Hrishikesh

AU - White, Luke A.

AU - Wang, Yuping

AU - Ballard, David H.

AU - Huebert, Robert C.

AU - Woerner, Jennifer E.

AU - Ghali, Ghali E.

AU - Alexander, Jonathan S.

N1 - Funding Information: This work was supported by the LSU Research and Technology Foundation. (LSU LIFT2 Grant 110351233A). DHB receives salary support from National Institutes of Health TOP-TIER grant T32-EB021955. Funding Information: Funding: This work was supported by the LSU Research and Technology Foundation. (LSU LIFT2 Grant 110351233A). DHB receives salary support from National Institutes of Health TOP-TIER grant T32-EB021955. Publisher Copyright: © 2019 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2019/1

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N2 - Three-dimensional (3D) printing is an additive manufacturing method that holds great potential in a variety of future patient-specific medical technologies. This project validated a novel crosslinked polyvinyl alcohol (XL-PVA) 3D printed stent infused with collagen, human placental mesenchymal stem cells (PMSCs), and cholangiocytes. The biofabrication method in the present study examined 3D printing and collagen injection molding for rapid prototyping of customized living biliary stents with clinical applications in the setting of malignant and benign bile duct obstructions. XL-PVA stents showed hydrophilic swelling and addition of radiocontrast to the stent matrix improved radiographic opacity. Collagen loaded with PMSCs contracted tightly around hydrophilic stents and dense choloangiocyte coatings were verified through histology and fluorescence microscopy. It is anticipated that design elements used in these stents may enable appropriate stent placement, provide protection of the stent-stem cell matrix against bile constituents, and potentially limit biofilm development. Overall, this approach may allow physicians to create personalized bio-integrating stents for use in biliary procedures and lays a foundation for new patient-specific stent fabrication techniques.

AB - Three-dimensional (3D) printing is an additive manufacturing method that holds great potential in a variety of future patient-specific medical technologies. This project validated a novel crosslinked polyvinyl alcohol (XL-PVA) 3D printed stent infused with collagen, human placental mesenchymal stem cells (PMSCs), and cholangiocytes. The biofabrication method in the present study examined 3D printing and collagen injection molding for rapid prototyping of customized living biliary stents with clinical applications in the setting of malignant and benign bile duct obstructions. XL-PVA stents showed hydrophilic swelling and addition of radiocontrast to the stent matrix improved radiographic opacity. Collagen loaded with PMSCs contracted tightly around hydrophilic stents and dense choloangiocyte coatings were verified through histology and fluorescence microscopy. It is anticipated that design elements used in these stents may enable appropriate stent placement, provide protection of the stent-stem cell matrix against bile constituents, and potentially limit biofilm development. Overall, this approach may allow physicians to create personalized bio-integrating stents for use in biliary procedures and lays a foundation for new patient-specific stent fabrication techniques.

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3D printing for bio-synthetic biliary stents

Abstract

Keywords

ASJC Scopus subject areas

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