3D porous chitosan-alginate scaffold stiffness promotes differential responses in prostate cancer cell lines

Kailei Xu, K. Ganapathy, Thomas Andl, Zi Wang, John A III Copland, R. Chakrabarti, Stephen J. Florczyk

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Prostate cancer (PCa) is a leading cause of death for men worldwide. Most PCa patients die from metastasis and bone is the most common metastatic site. Three dimensional (3D) porous chitosan-alginate (CA) scaffolds were developed for bone tissue engineering and demonstrated for culture of cancer cells and enrichment of cancer stem cells. However, only a single scaffold composition was studied. Three compositions of 3D porous CA scaffolds (2, 4, and 6 wt%) were used to investigate the effect of scaffold stiffness on PCa cell response with PC-3, C4-2B, and 22Rv1 cell lines. The PC-3 cells formed cell clusters while the C4-2B and 22Rv1 cells formed multicellular spheroids. The three cell lines demonstrated stiffness independent cell growth and expressed phenotypic PCa biomarkers. The osteoblastic PCa lines C4-2B and 22Rv1 mineralized in basal media, while the osteolytic PC-3 line did not, demonstrating that CA scaffold cultures revealed differences in PCa phenotypes. The CA scaffolds are a 3D culture platform that supports PCa growth and phenotypic expression with adjustable scaffold stiffness to mimic stages of metastatic progression. Further investigation of the scaffolds for co-culture of PCa cells with fibroblasts and primary PCa cell culture should be conducted to develop a platform for screening chemotherapies.

Original languageEnglish (US)
Article number119311
JournalBiomaterials
Volume217
DOIs
StatePublished - Oct 1 2019

Fingerprint

Alginate
Chitosan
Scaffolds
Prostatic Neoplasms
Cells
Stiffness
Cell Line
Cell culture
Scaffolds (biology)
Bone
Chemotherapy
Cell growth
Fibroblasts
Tumor Biomarkers
Cell Culture Techniques
Stem cells
Chemical analysis
Tissue engineering
Cellular Spheroids
alginic acid

Keywords

  • Mechanotransduction
  • Mineralization
  • Polyelectrolyte complex
  • Prostate cancer
  • Tumor microenvironment
  • Tumor model

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

3D porous chitosan-alginate scaffold stiffness promotes differential responses in prostate cancer cell lines. / Xu, Kailei; Ganapathy, K.; Andl, Thomas; Wang, Zi; Copland, John A III; Chakrabarti, R.; Florczyk, Stephen J.

In: Biomaterials, Vol. 217, 119311, 01.10.2019.

Research output: Contribution to journalArticle

Xu, Kailei ; Ganapathy, K. ; Andl, Thomas ; Wang, Zi ; Copland, John A III ; Chakrabarti, R. ; Florczyk, Stephen J. / 3D porous chitosan-alginate scaffold stiffness promotes differential responses in prostate cancer cell lines. In: Biomaterials. 2019 ; Vol. 217.
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